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Course Detail

Course Name Pharmaceutical Microbiology – Practical
Program Pharm. D.
Semester Three & Four
Year Taught 2014 , 2015 , 2016 , 2017 , 2018


‘Pharmaceutical Microbiology – Practical’ is a course offered in the second year of Pharm. D. program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.


Course Duration: 3 Hrs./Week

Lecture Wise Program

List of Experiments:

  1. Study of apparatus used in experimental microbiology*.
  2. Sterilisation of glass ware’s. Preparation of media and sterilisation.*
  3. Staining techniques – Simple staining ; Gram’s staining ; Negative staining**
  4. Study of motility characters*.
  5. Enumeration of micro-organisms (Total and Viable)*
  6. Study of the methods of isolation of pure culture.*
  7. Bio chemical testing for the identification of microorganisms.*
  8. Cultural sensitivity testing for some micro-organisms.*
  9. Sterility testing for powders and liquids.*
  10. Determination of minimum inhibitory concentration.*
  11. Microbiological assay of antibiotics by cup plate method.*
  12. Microbiological assay of vitamins by Turbidometric method**
  13. Determination of RWC.**
  14. Diagnostic tests for some common diseases, Widal, malarial parasite.**
    * Indicate minor experiment & ** indicate major experiment


Format of the assignment:

Scheme of Practical Examination

Sessionals Annual
Synopsis 05 15
Major Experiment 10 25
Minor Experiment 03 15
Viva 02 15
Max Marks 20 70
Duration 03 hrs 04 hrs

Note : Total sessional marks is 30 (20 for practical sessional plus 10 marks for regularity, promptness, viva-voce and record maintenance).

  1. Visit to some pathological laboratories & study the activities and equipment/instruments used and reporting the same.
  2. Visit to milk dairies (Pasteurization) and microbial laboratories (other sterilization methods) & study the activities and equipment/instruments used and reporting the same.
  3. Library assignments
    1. Report of recent microbial techniques developed in diagnosing some common diseases.
    2. Latest advancement developed in identifying, cultivating & handling of microorganisms.
    1. Minimum & Maximum number of pages.
    2. It shall be computer draft copy.
    3. Reference(s) shall be included at the end.
    4. Name and signature of the student.
    5. Assignment can be a combined presentation at the end of the academic year.
    6. Time allocated for presentation may be 8+2 Min.

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