‘Pharmacology II – Practical’ is a course offered in the third year of Pharm. D. program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.

Course Duration: 3 Hrs./Week

List of Experiments:

Title

1. Study of laboratory animals and their handling (a. Frogs, b. Mice, c. Rats, d. Guinea pigs, e. Rabbits).
2. Study of physiological salt solutions used in experimental pharmacology.
3. Study of laboratory appliances used in experimental pharmacology.
4. Study of use of anesthetics in laboratory animals.
5. To record the dose response curve of Ach using isolated ileum/rectus abdominis muscle preparation.
6. To carry out bioassay of Ach using isolated ileum/rectus abdominis muscle preparation by interpolation method.
7. To carry out bioassay of Ach using isolated ileum/rectus abdominis muscle preparation by three point method.
8. To record the dose response curve of Histamine using isolated guinea-pig ileum preparation.
9. Study of agonistic and antagonistic effects of drugs using isolated guinea-pig ileum preparation.
10. To carry out bioassay of Histamine using isolated guinea-pig ileum preparation by interpolation method.
11. To carry out bioassay of Histamine using guinea-pig ileum preparation by three point method.
12. To study the routes of administration of drugs in animals (Rats, Mice, Rabbits).
13. Study of theory, principle, procedure involved and interpretation of given results for the following ex- periments:
    1. Analgesic property of drug using analgesiometer.
    2. Antiinflammatory effect of drugs using rat-paw edema method.
    3. Anticonvulsant activity of drugs using maximal electroshock and pentylene tetrazole methods.
    4. Antidepressant activity of drugs using pole climbing apparatus and pentobarbitone induced sleeping time methods.
    5. Locomotor activity evaluation of drugs using actophotometer and rotorod.
    6. Cardiotonic activity of drugs using isolated frog heart and mammalian heart preparations.

Scheme of Practical Examination:

Note : Total sessional marks is 30 (20 for practical sessional plus 10 marks for regularity, promptness, viva-voce and record maintenance).