Back close

Isolation and Characterization of Host Binding Proteins from Bacillus Clausii Using Mass Spectrometry-a Proteomic Approach

Start Date: Sunday, Jan 01,2012

End Date: Wednesday, May 30,2012

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Dr. Sudarslal S.
Co-Project Incharge:Abitha L, Arjun S, Atish K, Clinton MU, Neethi S, Sanjay Pal
Isolation and Characterization of Host Binding Proteins from Bacillus Clausii Using Mass Spectrometry-a Proteomic Approach

Bacillus clausii, a gram positive, spore forming bacteria is widely used a probiotic in preventing and treating various gastrointestinal disorders. In order to understand the functioning of this strain as a probiotic, one needs to study the molecular level of interaction of this strain with host systems. Considering the fact that the first level of interaction can happen at the extracellular level, the project aims to characterize extracellular matrix proteins of Bacillus calusii. The workflow includes extraction, isolation and characterization of the extracellular proteins from Bacillus calusii using various analytical tools such as affinity chromatography, PAGE and mass spectrometry (MS). The MS based proteomic analysis revealed five surface proteins viz. UVRABC system protein, alkaline phosphatise, putative sugar-lactose lactonase, uncharacterized YRVC and endospore coat associated proteins. These proteins are thus important targets for elucidating the molecular cross talk between Bacillus clausii and their hosts.

Related Projects

Isolation and identification of endophytes from marine algae
Isolation and identification of endophytes from marine algae
Furfuryl Derivatives of Meldrum’s Acid: Synthesis, Characterisation and Biological Studies
Furfuryl Derivatives of Meldrum’s Acid: Synthesis, Characterisation and Biological Studies
Green Synthesis of Metal Nanoparticles & its Applications
Green Synthesis of Metal Nanoparticles & its Applications
Glucosamine Conjugated Chitosan Derivatives- Synthesis and Study of Antimicrobial Activity
Glucosamine Conjugated Chitosan Derivatives- Synthesis and Study of Antimicrobial Activity
Green synthesis of Metal Nano Particles like Gold or Silver and its Application
Green synthesis of Metal Nano Particles like Gold or Silver and its Application
Admissions Apply Now