Back close

Ribotyping of Fish Microflora

Start Date: Tuesday, Jan 01,2013

End Date: Thursday, May 30,2013

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Ajith Madhavan
Co-Project Incharge:AncyThaha,Jithin James , SharonD’Morris , SruthiChandran , Vishnu U L
Ribotyping of Fish Microflora

Microflora of fish intestines appear to vary with the complexity of the fish digestive system. The genera present in the gut generally seem to be those from the environment or diet which can survive and multiply in the intestinal tract. The main aim of the work is to identify the organism present in the gut of fish through ribotyping technique. To start up with the procedure first standardized the ribotyping technique. For standardization of the procedure a gram+ve(Bacillus) and a gram-ve (E.coli) was used. Genomic DNA was successfully isolated from both organisms. Hot shot method was performed. PCR method was standardized. As the procedure standardized the microflora present in the fish gut was successfully isolated by serial dilution. Sixteen morphologically different colonies were got. DNA was isolated using hotshot method. Extracted DNA was subjected to PCR amplification. Four samples showed strong bands and send for sequencing.

Related Projects

Effect of Environmental Factors on Klebsiella Biofilm Formation
Effect of Environmental Factors on Klebsiella Biofilm Formation
Determination of Antibacterial Activity of Different Spices Against E.coli
Determination of Antibacterial Activity of Different Spices Against E.coli
Screening, Identification and Characterisation of Bacteriocins Isolated from Wine Microflora
Screening, Identification and Characterisation of Bacteriocins Isolated from Wine Microflora
Isolation and Characterization of Host Binding Proteins from Bacillus Clausii Using Mass Spectrometry-a Proteomic Approach
Isolation and Characterization of Host Binding Proteins from Bacillus Clausii Using Mass Spectrometry-a Proteomic Approach
Screening for Compounds with Lifespan Altering Effect on Caenorhabditis Elegans and Gene Expression Microarray Data Based Computational Drug Repurposing
Screening for Compounds with Lifespan Altering Effect on Caenorhabditis Elegans and Gene Expression Microarray Data Based Computational Drug Repurposing
Admissions Apply Now