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5-Flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Journal of Biomedical Nanotechnology

Source : Journal of Biomedical Nanotechnology, Volume 8, Number 1, p.29-42 (2012)

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Keywords : 5-fluorouracil, 5-fluorouracil (5-FU), animal cell, Animals, Anticancer activities, Antimetabolites, Antineoplastic, antineoplastic activity, apoptosis, article, Assays, Biomaterials, Biomedical fields, Biopolymers, Blood Coagulation Tests, blood compatibility, breast cancer, Breast cancer cells, Breast Neoplasms, cancer chemotherapy, Carboxymethyl, caspase 3, Caspase-3, Cell death, Cell Line, Cell Survival, Cellular internalization, Chemotherapy, chitosan, Coagulation, Coagulation assays, controlled study, cytotoxicity, Diseases, Drug Carriers, Drug delivery, drug delivery system, Drug entrapment, Drug loaded nanoparticle, drug release, female, flow cytometry, Flow-cytometric analysis, fluorescence microscopy, Fluorescent microscopy, fluorouracil, Hemolysis, Hemolytic assays, hemolytic plaque assay, Humans, In-vitro, Mean diameter, Medical nanotechnology, Mice, mouse, n, nanomedicine, nanoparticle, Nanoparticles, nonhuman, o carboxymethyl chitosan

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2012

Abstract : Chitosan and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, biodegradable and hence found applications in biomedical field. In the current work, we have developed 5-fluorouracil (5-FU) loaded N,O-carboxymethyl chitosan (N,O-CMC) nanoparticles (mean diameter: 80±20 nm, zeta potential: +52±47±2 mV) for cancer drug delivery. Drug entrapment efficiency (65%) and in vitro drug release studies were carried out spectrophotometricaly. Cellular internalization of the drug loaded nanoparticles was confirmed by fluorescent microscopy and flow cytometric analysis. Results of anticancer activity via MTT, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. As a whole these results indicates the potential of 5-FU loaded N,O-CMC nanoparticles in breast cancer chemotherapy in which the side effects of conventional chemo treatment could be reduced. Furthermore, the results of in vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system as well. Copyright © 2012 American Scientific Publishers All rights reserved.

Cite this Research Publication : A. Anitha, Chennazhi, K. P., Nair, S. V., and Dr. Jayakumar Rangasamy, “5-Flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer”, Journal of Biomedical Nanotechnology, vol. 8, pp. 29-42, 2012.

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