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Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Journal of Biomedical Nanotechnology
Source : Journal of Biomedical Nanotechnology, Volume 8, Number 1, p.29-42 (2012)
Keywords : 5-fluorouracil, 5-fluorouracil (5-FU), animal cell, Animals, Anticancer activities, Antimetabolites, Antineoplastic, antineoplastic activity, apoptosis, article, Assays, Biomaterials, Biomedical fields, Biopolymers, Blood Coagulation Tests, blood compatibility, breast cancer, Breast cancer cells, Breast Neoplasms, cancer chemotherapy, Carboxymethyl, caspase 3, Caspase-3, Cell death, Cell Line, Cell Survival, Cellular internalization, Chemotherapy, chitosan, Coagulation, Coagulation assays, controlled study, cytotoxicity, Diseases, Drug Carriers, Drug delivery, drug delivery system, Drug entrapment, Drug loaded nanoparticle, drug release, female, flow cytometry, Flow-cytometric analysis, fluorescence microscopy, Fluorescent microscopy, fluorouracil, Hemolysis, Hemolytic assays, hemolytic plaque assay, Humans, In-vitro, Mean diameter, Medical nanotechnology, Mice, mouse, n, nanomedicine, nanoparticle, Nanoparticles, nonhuman, o carboxymethyl chitosan
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2012
Abstract : Chitosan and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, biodegradable and hence found applications in biomedical field. In the current work, we have developed 5-fluorouracil (5-FU) loaded N,O-carboxymethyl chitosan (N,O-CMC) nanoparticles (mean diameter: 80±20 nm, zeta potential: +52±47±2 mV) for cancer drug delivery. Drug entrapment efficiency (65%) and in vitro drug release studies were carried out spectrophotometricaly. Cellular internalization of the drug loaded nanoparticles was confirmed by fluorescent microscopy and flow cytometric analysis. Results of anticancer activity via MTT, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. As a whole these results indicates the potential of 5-FU loaded N,O-CMC nanoparticles in breast cancer chemotherapy in which the side effects of conventional chemo treatment could be reduced. Furthermore, the results of in vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system as well. Copyright © 2012 American Scientific Publishers All rights reserved.
Cite this Research Publication : A. Anitha, Chennazhi, K. P., Nair, S. V., and Dr. Jayakumar Rangasamy, “5-Flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer”, Journal of Biomedical Nanotechnology, vol. 8, pp. 29-42, 2012.