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A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells.

Publication Type : Journal Article

Publisher : Chemical Communications

Source : Chemical Communications, Royal Society of Chemistry, Volume 53, Issue 81, p.11213-11216 (2017)

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Keywords : Animals, Boron Compounds, Cell Line, Tumor, Cell Survival, Fluorescence, Fluorescent Dyes, Hela Cells, Humans, hydrogen peroxide, Mice, Microscopy, Fluorescence, Multiphoton, Molecular Structure, Monophenol Monooxygenase, Optical imaging, Photons, Reproducibility of Results, Up-Regulation

Campus : Coimbatore

School : School of Engineering

Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)

Department : Civil

Year : 2017

Abstract : Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λ = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about HO-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.

Cite this Research Publication : K. Naidu Bobba, Won, M., Shim, I., Velusamy, N., Yang, Z., Qu, J., Kim, J. Seung, and Bhuniya, S., “A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells.”, Chemical Communications, vol. 53, no. 81, pp. 11213-11216, 2017.

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