Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : International Journal of Biological Macromolecules
Source : International Journal of Biological Macromolecules, Volume 110 (2017)
Keywords : Anti-septic, Benzalkonium chloride (BZK), ChitosanHyaluronic acid (HA), Gelatin nanoparticles, Gelatin zymography, Matrix metalloprotease (MMP), Sodium alendronate
Campus : Kochi
School : Center for Nanosciences, School of Medicine
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine, General Surgery
Year : 2017, 2018
Abstract : Chronic diabetic wounds is characterised by increased microbial contamination and overproduction of matrix metalloproteases that would degrade the extracellular matrix. A bi-layer bandage was developed, that promotes the inhibition of microbial infections and matrix metalloprotease (MMPs) activity. Bi-layer bandage containing benzalkonium chloride loaded gelatin nanoparticles (BZK GNPs) in chitosan-Hyaluronic acid (HA) as a bottom layer and sodium alendronate containing chitosan as top layer was developed. We hypothesized that the chitosan-gelatin top layer with sodium alendronate could inhibit the MMPs activity, whereas the chitosan-HA bottom layer with BZK GNPs (240 ± 66 nm) would enable the elimination of microbes. The porosity, swelling and degradation nature of the prepared Bi-layered bandage was studied. The bottom layer could degrade within 4 days whereas the top layer remained upto 7 days. The antimicrobial activity of the BZK NPs loaded bandage was determined using normal and clinical strains. Gelatin zymography shows that the proteolytic activity of MMP was inhibited by the bandage.
Cite this Research Publication : A. Jayasree, Mohandas, A., Seethalakshmy, S., Suresh, M., Riju R. Menon, Biswas, R., and Jayakumar, R., “Bi-layered nanocomposite bandages for controlling microbial infections and overproduction of matrix metalloproteinase activity”, International Journal of Biological Macromolecules, vol. 110, 2017.