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Publication Type : Journal Article
Thematic Areas : Medical Sciences, Nanosciences and Molecular Medicine
Publisher : ACS Sustainable Chemistry and Engineering, American Chemical Society
Source : ACS Sustainable Chemistry and Engineering, American Chemical Society, Volume 6, Number 6, p.7826-7840 (2018)
Keywords : Anti-bacterial activity, Bacterial Infections, Cardiovascular surgery, chitin, Endothelial cells, Escherichia coli, Escherichia coli (E. coli), Gelatin nanoparticles, Human umbilical vein endothelial cells, Methicillin-Resistant Staphylococcus aureus, Mixtures, Sprague-Dawley rats, Staphylococcus aureus, Syringes, Transplantation (surgical)
Campus : Kochi
School : Center for Nanosciences, School of Medicine
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine, Microbiology, C. V. T. S.
Year : 2018
Abstract : Mediastinitis occurs after cardiac surgery and is a major threat to patient's life due to postoperative bleeding and deep sternal wound infection. Major challenge in treating this condition is that it demands a material that should adhere to the applied site and act as both a hemostatic and an antibacterial agent. On the basis of this we have developed an in situ forming tissue adhesive chitin-fibrin (CH-FB) gel with tigecycline loaded gelatin nanoparticles (tGNPs) for controlling bleeding and preventing bacterial infection. Spherical shaped tGNPs (231 ± 20 nm) were prepared and characterized. In situ forming tGNPsCH-FB gel was formed using a dual syringe applicator in which one syringe was loaded with a mixer of fibrinogen solution, chitin gel, and tGNPs; the other syringe was loaded with a mixture of thrombin solution, chitin gel, and tGNPs. Both these mixtures were injected together. In situ gel formed within a minute and exhibited excellent tissue adhesive property. tGNPsCH-FB gel was found to be cyto-compatible against human umbilical vein endothelial cells (HUVECs). Sustained release of tigecycline from tGNPsCH-FB gel was found to occur over 21 days. In vitro antibacterial activity of tGNPsCH-FB gel was tested against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli (E. coli), and their clinical isolates. Furthermore, in vivo hemostatic potential of tGNPsCH-FB gel was evaluated in deep organ injuries created in Sprague-Dawley rats. The developed gel exhibited rapid blood clotting potential by achieving hemostasis within 154 and 84 s under femoral artery (pressured) and liver (oozing) bleeding conditions. Hence, these findings exhibit the potential application of the developed tGNPsCH-FB gel to adhere at surgical site for controlling bleeding and prevent bacterial infection after cardiac surgery. © 2018 American Chemical Society.
Cite this Research Publication : Nivedhitha Sundaram M., V. Kaliannagounder, K., Selvaprithiviraj, V., Suresh, M., Biswas, R., Vasudevan, A. K., Dr. Praveen Varma, and Dr. Jayakumar Rangasamy, “Bioadhesive, Hemostatic, and Antibacterial in Situ Chitin-Fibrin Nanocomposite Gel for Controlling Bleeding and Preventing Infections at Mediastinum”, ACS Sustainable Chemistry and Engineering, vol. 6, pp. 7826-7840, 2018.