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Cell mimetic monolayer supported chitosan-haemocompatibility studies

Publication Type : Journal Article

Publisher : Wiley Online Library

Source : Journal of Biomedical Materials Research Part A, Wiley Online Library, Volume 79, Issue 1, p.147–152 (2006)

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Keywords : Calcification, chitosan, Haemocompatibility, monolayer, phosphatidylcholine

Campus : Kochi

School : School of Pharmacy

Center : Amrita Institute of Medical Science

Department : Pharmaceutics

Verified : Yes

Year : 2006

Abstract : Chitosan is a natural polymer, widely explored for biomedical and tissue engineering applications. However the thrombogenic nature limits their application in blood contacting devices and implants. Here, we have attempted to understand the haemocompatibility of chitosan by immobilizing a monolayer of cell mimetic lipid compositions. The phosphatidylcholine/cholesterol/galactocerebroside lipid composition (PC/Chol/GalC, 1:0.35:0.125) was deposited onto the chitosan films. Characterization of the modified surface was done by sessile drop contact angle measurement. The contact angle of the chitosan film reduced from 80.65 ± 1.4 to 23.5 ± 1.9 after the surface modification. Swelling nature of chitosan seemed to influence the orientation and packing of the lipid monolayer. In vitro calcification studies with metastable salt solution indicated increased calcification on the modified surface. This may be due to formation of nuclei for calcification on the expanding monolayer. The preliminary haemocompatibility studies with washed platelets, leukocytes and erythrocytes showed overall reduction in blood cell adhesion to the modified surfaces. Scanning electron microscopy was used for morphological characterization of platelet adhesion and activation on the surfaces. On the bare chitosan surface, fully spread platelets with extending pseudopodia indicated platelet activation. The smooth surface of the modified film did not activate platelets. These studies showed that, though the lipid monolayer on chitosan film is able to reduce the over all blood cell adhesion and platelet activation it is prone to calcification. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

Cite this Research Publication : S. Mathews, Dr. Kaladhar Kamalasanan, and Sharma, C. P., “Cell mimetic monolayer supported chitosan-haemocompatibility studies”, Journal of Biomedical Materials Research Part A, vol. 79, no. 1, pp. 147–152, 2006.

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