Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Cancer Res
Source : Cancer Res, Volume 77, Issue 11, p.2914-2926 (2017)
Url : https://www.ncbi.nlm.nih.gov/pubmed/28377454
Keywords : 5'-Nucleotidase, Aneuploidy, Animals, Cell Line, Tumor, Disease Models, Animal, Humans, Mice, Prion Proteins, signal transduction, Stress, Physiological
Campus : Amritapuri
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2017
Abstract : Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73 cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrP was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. .
Cite this Research Publication : P. H. Domingues, S Y Nanduri, L., Seget, K., Venkateswaran, S. V., Agorku, D., Viganó, C., von Schubert, C., Nigg, E. A., Swanton, C., Sotillo, R., Bosio, A., Storchová, Z., and Hardt, O., “Cellular Prion Protein PrP and Ecto-5'-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy.”, Cancer Res, vol. 77, no. 11, pp. 2914-2926, 2017.