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Characterization of the bacteriophages binding to human matrix molecules

Publication Type : Journal Article

Thematic Areas : Biotech

Publisher : International Journal of Biological Macromolecules

Source : International Journal of Biological Macromolecules 110: 179-184.

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Keywords : Bacteriophages, Cell Line, Tumor, colon, Escherichia coli, Escherichia coli Infections, Extracellular Matrix Proteins, Humans

Campus : Amritapuri

School : School of Biotechnology

Center : Biotechnology, Sanitation Biotechnology

Department : biotechnology

Year : 2018

Abstract : Recent literature has suggested a novel symbiotic relationship between bacteriophage and metazoan host that provides antimicrobial defense protecting mucosal surface by binding to host matrix mucin glycoproteins. Here, we isolated and studied different bacteriophages that specifically interact with human extracellular matrix molecules such as fibronectin, gelatin, heparin and demonstrated their potency for protection to host against microbial infections. We showed that subpopulations of bacteriophages that work against clinical isolates of Escherichia coli can bind to pure gelatin, fibronectin and heparin and reduced bacterial load in human colon cell line HT29. The bacteriophages were characterized with respect to their genome sizes, melting curve patterns and host tropism (cross-reactivity with different hosts). Since, the bacteriophages are non-toxic to the host and can effectively reduce bacterial load in HT29 cell line their therapeutic potency against bacterial infection could be explored

Cite this Research Publication : C. Porayath, Salim, A., Veedu, A. Palillam, Babu, P., Nair, B., Madhavan, A., and Pal, S., “Characterization of the bacteriophages binding to human matrix molecules.”, Int J Biol Macromol, vol. 110, pp. 608-615, 2018.

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