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Computational Approach for Identifying Therapeutic Micro RNAs

Publication Type : Journal Article

Publisher : International Journal of Pharmacy and Pharmaceutical Sciences

Source : International Journal of Pharmacy and Pharmaceutical Sciences

Url :

Campus : Coimbatore

Center : Computational Engineering and Networking

Year : 2014

Abstract : Objective: Identification of novel micro RNAs (miRNAs) in treating diseases has become a challenge in the era of post genomics and the ability to apply an accurate computational approach leads to the discovery of therapeutic miRNAs to treat diseases. Methods: Initially we have identified the list of list of genes from PharmGkB Results: We have applied the above mentioned approach to Psoriasis. In case of Psoriasis, 29 genes are present in pharmacogenomic database and among those 29 genes only IL6, ABCC1, VDR and ABCG2 contain validated miRNAs with strong experimental evidence in miRTarBase. Binding sites were analyzed for the obtained miRNAs and it has been found that hsa-let-7a-5p, hsa-miR-26a-5p, hsa-miR-345-5p, hsa-miR-125b-5p, hsa-let-7a-5p, hsa-miR-27b-3p and hsa-miR-328-3p contain 1 binding site. Similarly hsa-miR-365a-3p, hsa-miR-7-5p, hsa-miR-519c-3p and hsa-miR-520h contain 2 binding sites. and then we have identified the validated miRNA targets from miRTarBase. Finally we have found the connectivity map between the gene and validated miRNA target from miRmap and the number of binding sites were analyzed for each pair (gene-miRNA). Conclusion: Since hsa-miR-365a-3p of IL6, hsa-miR-7-5p of ABCC1, hsa-miR-519c-3p and hsa-miR-520h of ABCG2 contain two binding sites, these miRNAs have a maximum probability to become a therapeutic target of Psoriasis in future and the above mentioned methodology can also be applied for other diseases.

Cite this Research Publication : A Harishchander, DA Anand, Computational approach for identifying therapeutic micro RNAs - International Journal of Pharmacy and Pharmaceutical, 2014

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