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Curcumin-loaded N, O-carboxymethyl chitosan nanoparticles for cancer drug delivery

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Journal of Biomaterials Science, Polymer Edition

Source : Journal of Biomaterials Science, Polymer Edition, Volume 23, Number 11, p.1381-1400 (2012)

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Keywords : AFM, animal cell, Anti-inflammatories, Anti-microbial effects, Anti-proliferative, apoptosis, article, atomic force microscopy, Biochemistry, Biomedical applications, Biopolymers, cancer cell, Cancer cells, Carboxymethyl, Carboxymethyl chitosan, cell structure, cell viability, Cells, Cellular uptake, Chemotherapeutic agents, chitosan, controlled study, curcumin, cytotoxicity, Drug delivery, drug delivery system, drug formulation, drug release, Dynamic light scattering, flow cytometry, fluorescence microscopy, Hemolysis, human, human cell, in vitro study, In-vitro, Incubation periods, infrared spectroscopy, lactate dehydrogenase, light scattering, lysozyme, Mean diameter, Medical applications, mouse, MTT assays, n, Nanocarriers, nanoencapsulation, Nanoformulation, nanoparticle, Nanoparticles, Non-toxicity, nonhuman, o carboxymethy chitosan, O-carboxymethyl chitosans, particle size, pH, priority journal, scanning electron microscopy

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2012

Abstract : Chitosan (CS) and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible and biodegradable, and, hence, suitable for various biomedical applications, such as drug delivery, gene therapy and tissue engineering. Curcumin is a major chemotherapeutic agent with antioxidant, antiinflammatory, anti-proliferative, anticancer and antimicrobial effects. However, the potential of curcumin as a chemotherapeutic agent is limited by its hydrophobicity and poor bioavailability. In this work, we developed a nanoformulation of curcumin in a carboxymethyl chitosan (CMC) derivative, N, O -carboxymethyl chitosan (N,O-CMC). The curcumin-loaded N,O-CMC (curcumin-N,O-CMC) nanoparticles were characterized using DLS, AFM, SEM, FT-IR and XRD. DLS studies revealed nanoparticles with a mean diameter of 150 ± 30 nm. AFM and SEM confirmed that the particles have a spherical morphology within the size range of 150 ± 30 nm. Curcumin was entrapped with in N,O-CMC nanopartcles with an efficiency of 80%. The in vitro drug-release profile was studied at different pH (7.4 and 4.5) at 37°C for different incubation periods with and without lysozyme. Cytotoxicity studies using MTT assay indicated that curcumin-N,O-CMC nanoparticles showed specific toxicity towards cancer cells and non-toxicity to normal cells. Cellular uptake of curcumin-N,O-CMC nanoparticles was analyzed by fluorescence microscopy and was reconfirmed by flow cytometry. Overall, these results indicate that like previously reported curcumin loaded O-CMC nanoparticles, N, O-CMC will also be an efficient nanocarrier for delivering curcumin to cancer cells. © 2011 Koninklijke Brill NV, Leiden.

Cite this Research Publication : A. Anitha, Maya, S., Deepa, N., Chennazhi, K. P., Nair, S. V., and Dr. Jayakumar Rangasamy, “Curcumin-loaded N, O-carboxymethyl chitosan nanoparticles for cancer drug delivery”, Journal of Biomaterials Science, Polymer Edition, vol. 23, pp. 1381-1400, 2012.

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