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Detergent compatible assay for protein estimation

Publication Type : Patents

Publisher : Amrita Vishwa Vidyapeetham (Kollam, IN)

Source : Amrita Vishwa Vidyapeetham (Kollam, IN) , Volume United States Patent Application No. US 2019/0219591 , Number 16/366865 , India (2019)

Url : https://www.freepatentsonline.com/y2019/0219591.html

Campus : Amritapuri

School : School of Biotechnology

Department : biotechnology

Year : 2018, 2019

Abstract : The invention discloses a detergent-compatible protein assay method, composition and kit based on bio-conjugation reaction between protein and Meldrum's acid activated furfural. The method includes adding MAF in dimethyl sulfoxide (DMSO) to a protein sample solution. The amine functionalities present on the amino acid residues reacts with the MAF instantaneously at room temperature to yield deep purple colored solutions of the corresponding conjugated proteins. The reagent composition added to protein may be in the range of 90-450 mM. The intensities of purple colored solutions were proportional to the protein concentration captured by spectrophotometric measurements. The assay is sensitive in the range of 0.125-15 mg/mL, is compatible with commonly used detergents and reducing agents in protein solutions and may be employed for estimation of protein samples in the presence of detergents and reducing agents.

Cite this Research Publication : Dr. Sobha V. Nair, Nair, P. Chandran R., Nair, B., and Ajayan, K., “Detergent Compatible Assay for Protein Estimation”, U.S. Patent 16/366865 2019

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