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Electrospinning of Carboxymethyl Chitin/poly(vinyl alcohol) Nanofibrous Scaffolds for Tissue Engineering Applications

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Carbohydrate Polymers

Source : Carbohydrate Polymers, Volume 77, Number 4, p.863 - 869 (2009)

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Keywords : Bioactive, Caboxymethyl chitin, Electrospinning, Nanofibrous scaffold, tissue engineering

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2009

Abstract : A novel fibrous membrane of carboxymethyl chitin (CMC)/poly(vinyl alcohol) (PVA) blend was successfully prepared by electrospinning technique. The concentration of CMC (7%) with PVA (8%) was optimized, blended in different ratios (0–100%) and electrospun to get nanofibers. Fibers were made water insoluble by chemical followed by thermal cross-linking. In vitro mineralization studies identified the ability of formation of hydroxyapatite deposits on the nanofibrous surfaces. Cytotoxicity of the nanofibrous scaffold was evaluated using human mesenchymal stem cells (hMSCs) by the MTT assays. The cell viability was not altered when these nanofibrous scaffolds were pre-washed with phosphate buffer containing saline (PBS) before seeding the cells. The SEM images also revealed that cells were able to attach and spread in the nanofibrous scaffolds. Thus our results indicate that the nanofibrous CMC/PVA scaffold supports cell adhesion/attachment and proliferation and hence this scaffold will be a promising candidate for tissue engineering applications.

Cite this Research Publication : K. T. Shalumon, Binulal, N. S., Selvamurugan, N., Nair, S. V., Menon, D., Furuike, T., Tamura, H., and Dr. Jayakumar Rangasamy, “Electrospinning of Carboxymethyl Chitin/poly(vinyl alcohol) Nanofibrous Scaffolds for Tissue Engineering Applications”, Carbohydrate Polymers, vol. 77, pp. 863 - 869, 2009.

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