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Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions – an in vitro study

Publication Type : Journal Article

Publisher : Dental Traumatology

Source : Dental Traumatology, Volume 27, Number 3, p.217-220 (2011)

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Keywords : animal, Animals, article, cell count, Cell Culture Techniques, Cell Survival, Clinical trial, coloring agent, Coloring Agents, comparative study, controlled clinical trial, controlled study, culture technique, Cytology, desiccation, diagnostic agent, drug effect, Hanks Balanced Salt Solution, human, Humans, isotonic solution, Isotonic Solutions, Materials Testing, Milk, oral rehydration solution, Organ Preservation Solutions, pathology, periodontal ligament, preservation solution, randomized controlled trial, Rehydration Solutions, time, Time Factors, Tooth Avulsion, tooth injury, trypan blue

Campus : Kochi

School : School of Dentistry

Department : Conservative Dentistry and Endodontics

Year : 2011

Abstract : The prognosis of teeth replanted following avulsion is determined by the extra-alveolar time and storage medium used. This study was undertaken to determine the efficacy of an oral rehydration solution 'Ricetral', in retaining the vitality of periodontal ligament cells when used as a storage medium for avulsed teeth prior to replantation. The study consisted of a comparative evaluation between Ricetral and two currently recommended solutions, Hank's balanced salt solution (HBSS) and milk. Thirty extracted teeth were dried for 30min and soaked in the respective storage media for 45min. The periodontal ligament cells were isolated by an enzyme treatment with collagenase and trypsin. The cells were evaluated for vitality by trypan blue staining and number of vital cells counted in a hemocytometer. Statistical analysis revealed that cell vitality was high with Ricetral and HBSS, but poor with milk. © 2011 John Wiley amp; Sons A/S.

Cite this Research Publication : Pa Rajendran, Varghese, N. Ob, Varughese, J. Mb, and Murugaian, Ec, “Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions - an in vitro study”, Dental Traumatology, vol. 27, pp. 217-220, 2011.

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