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Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Gene Expression Patterns, Elsevier,

Source : Gene Expression Patterns, Elsevier, Volume 10, Number 6, p.283–289 (2010)

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Keywords : Gsdf; Dmy; Sexual differentiation; Estradiol-17β; Sex reversal; Medaka; TGF-β superfamily; Cytokines

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2010

Abstract : In the teleost fish, medaka (Oryzias latipes), the sex is genetically determined at the time of fertilization. The males are heterogametic with XYnbsp;chromosomenbsp;composition, while females are of XX chromosome composition. The male sexual differentiation is initiated in XY embryos of medaka by the sex-determining genenbsp;Dmy. In this study, we have cloned the gonadal soma derived factor (Gsdf) from medaka and characterized its expression pattern during the initiation of morphological testicular differentiation. Bynbsp;real-time PCR, an XY-specificnbsp;up-regulationnbsp;was detected in the expression levels ofnbsp;Gsdfnbsp;in the whole embryos of medaka at 6nbsp;days post fertilization (dpf), coincident with the initiation of testicular differentiation in the XY gonads. Whole mount and sectionnbsp;in situ hybridizationsnbsp;reaffirmed thatnbsp;Gsdfnbsp;was expressed exclusively in primordial gonads of only the genetic males at 6nbsp;dpf. Conversely, the expression ofnbsp;Gsdfnbsp;was found to be very weak in the XX gonads during embryogenesis. Importantly,nbsp;Gsdfnbsp;andnbsp;Dmynbsp;were found to be co-localized in the samesomatic cellsnbsp;in the XY gonads. When the XY embryos were treated with estradiol-17β, in order to reverse theirnbsp;phenotypicnbsp;sex, a decline was observed in the expression ofnbsp;Gsdfnbsp;in these embryos by real-time PCR.

Cite this Research Publication : Y. Shibata, Dr. Bindhu Paul, Suzuki, A., Usami, T., Nakamoto, M., Matsuda, M., and Nagahama, Y., “Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka”, Gene Expression Patterns, vol. 10, pp. 283–289, 2010.

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