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Fabrication of chitin/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) hydrogel scaffold

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Carbohydrate Polymers

Source : Carbohydrate Polymers, Volume 90, Number 1, p.725-729 (2012)

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Keywords : Blend hydrogels, Blending, Cell attachments, Cell culture, Cell number, chitin, Composite materials, Cytocompatibility, Human dermal fibroblasts, Hydrogel scaffolds, Hydrogels, Macroporous, Normal solutions, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), Scaffolds, Scaffolds (biology), Skin tissue, Skin tissue engineering, Tissue

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2012

Abstract : Regeneration of skin after a large area wound or burn is often difficult without the aid of a scaffold. In this work we developed a 3D macroporous chitin/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) hydrogel blend by normal solution chemistry and lyophilized it to get the scaffold. The developed scaffold was then characterized and it showed a slow degrading as well as biocompatible nature. The blend hydrogel scaffold showed 67% porosity and the pore size was less than 20 μm. Cytocompatibility of the scaffold was proved by Alamar blue assay using Human Dermal Fibroblast cells (HDF). The blend hydrogel scaffold showed a two fold increase in cell number over control within three days of culture. The chitin/PHBV blend also showed enhanced HDF cell attachment and proliferation. These preliminary results prove its suitability for scaffold based skin tissue regeneration. © 2012 Elsevier Ltd.

Cite this Research Publication : D. Sankar, Chennazhi, K. P., Nair, S. V., and Dr. Jayakumar Rangasamy, “Fabrication of chitin/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) hydrogel scaffold”, Carbohydrate Polymers, vol. 90, pp. 725-729, 2012.

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