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Fluorescence estimation of total protein in blood serum using N-doped carbon dots

Publication Type : Journal Article

Thematic Areas : Biotech

Publisher : Materials Today: Proceedings

Source : Materials Today: Proceedings, Volume 33, p.2233-2237 (2020)

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Keywords : Fluorescence assay, Fluorescence enhancement, Hydrophobic interaction, N-doped carbon dots, Total protein

Campus : Amritapuri, Coimbatore

School : School of Biotechnology, School of Engineering

Center : Biosensor Research Lab, Biotechnology

Department : biotechnology, Sciences

Year : 2020

Abstract : In this article, Nitrogen-doped carbon dots (NCDs) were used as a fluorescent probe for the detection of total serum proteins. NCDs were synthesized by microwave-assisted method using citric acid as the carbon source and ethylenediamine as passivating agent. The strong intensity of fluorescence emission was observed at λex/λem ≈ 350/460 nm. The particles are found to be uniformly spherical with an average dimension of 10 nm. The fluorescence intensity of NCDs increased linearly with the addition of bovine serum albumin (BSA) concentrations from 0.5 g/dL to 15 g/dL. The role of interfering agents was investigated and found that NCDs were sensitive to BSA specifically.

Cite this Research Publication : R. Anjali, Pathak, A., Suneesh, P. V., and Dr. Satheesh Babu T. G., “Fluorescence estimation of total protein in blood serum using N-doped carbon dots”, Materials Today: Proceedings, vol. 33, pp. 2233-2237, 2020.

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