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Publication Type : Journal Article
Thematic Areas : Biotech
Publisher : Materials Today: Proceedings
Source : Materials Today: Proceedings, Volume 33, p.2233-2237 (2020)
Keywords : Fluorescence assay, Fluorescence enhancement, Hydrophobic interaction, N-doped carbon dots, Total protein
Campus : Amritapuri, Coimbatore
School : School of Biotechnology, School of Engineering
Center : Biosensor Research Lab, Biotechnology
Department : biotechnology, Sciences
Year : 2020
Abstract : In this article, Nitrogen-doped carbon dots (NCDs) were used as a fluorescent probe for the detection of total serum proteins. NCDs were synthesized by microwave-assisted method using citric acid as the carbon source and ethylenediamine as passivating agent. The strong intensity of fluorescence emission was observed at λex/λem ≈ 350/460 nm. The particles are found to be uniformly spherical with an average dimension of 10 nm. The fluorescence intensity of NCDs increased linearly with the addition of bovine serum albumin (BSA) concentrations from 0.5 g/dL to 15 g/dL. The role of interfering agents was investigated and found that NCDs were sensitive to BSA specifically.
Cite this Research Publication : R. Anjali, Pathak, A., Suneesh, P. V., and Dr. Satheesh Babu T. G., “Fluorescence estimation of total protein in blood serum using N-doped carbon dots”, Materials Today: Proceedings, vol. 33, pp. 2233-2237, 2020.