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FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H 2 O 2 /H 2 S formation through multicolor images

Publication Type : Journal Article

Thematic Areas : Advanced Materials and Green Technologies

Publisher : Journal of Photochemistry and Photobiology B: Biology

Source : Journal of Photochemistry and Photobiology B: Biology

Campus : Coimbatore

School : School of Engineering

Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)

Department : Civil

Year : 2019

Abstract : We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H 2 S and H 2 O 2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λ abs 390 nm) and fluorescence emission (λ em 460 nm) of probe PHS1 in the presence of H 2 O 2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H 2 S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H 2 O 2 , H 2 S, and H 2 O 2 /H 2 S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H 2 S, H 2 O 2 ) in cancer cells. © 2018

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