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Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Journal of Materials Chemistry
Source : Journal of Materials Chemistry, Volume 22, Number 5, p.1904-1915 (2012)
Keywords : 3D architectures, Adhesion, Alkaline phosphatase activity, Alkalinity, Biomimetic Materials, Biomimetics, Bone, Bone matrix, Bone regeneration, Bone-like tissue, Cell culture, Chemical cross-linking, Coated Materials, Crosslinking, Growth (materials), Human mesenchymal stem cells (hMSCs), matrix, Metallic titanium, Microporosity, Microporous, Molecules, Nanofibers, Natural polymers, Orthopedic implant, Osteogenic, Osteointegration, Phosphatases, Scaffolds, Scaffolds (biology), Stem cells, Surfaces, Three dimensional, Tissue, Titanium, Titanium surfaces
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2012
Abstract : One major weakness that all orthopedic implants have in common is the limited osteointegration which results in post-implant loosening. Though biomimetic and biospecific molecules coated on materials were shown to enhance the function of cells, the 3D organization and spatial distribution of these molecules play a critical role in controlling adhesion, growth, viability, differentiation, and function of the cells. Here, we report an approach of surface scaffolding on metallic titanium (Ti) using bioactive natural polymers to enhance osteointegration and bone regeneration as an alternative to surface modification. Instead of just depositing the biomimetic molecules, we constructed a 3D scaffolding of fibrin and alginate on metallic Ti by a method involving chemical crosslinking with dopamine followed by lyophilization. The developed microporous-nanofibrous scaffold showed close resemblance to the native bone matrix in terms of the 3D architecture and was found to be firmly attached to the Ti surfaces. The effectiveness of the obtained scaffold to act as a better matrix for the attachment and proliferation of stem cells and their differentiation into osteogenic lineage was studied using human Mesenchymal Stem Cells (hMSCs). The alkaline phosphatase (ALP) activity and subsequent mineralization of the scaffolds by the differentiated osteoblasts were enhanced greatly by the immobilized scaffold on Ti, eventually leading to bone-like tissue formation over the surface within 2-3 weeks. Thus, the generation of biomimetic 3D scaffolding on Ti surface seems to be a potential approach for improving osteointegration of orthopedic implants in lieu of or in combination with surface modification. © 2012 The Royal Society of Chemistry.
Cite this Research Publication : S. Soumya, Sreerekha, P. R., Dr. Deepthy Menon, Nair, S. V., and Chennazhi, K. P., “Generation of a biomimetic 3D microporous nano-fibrous scaffold on titanium surfaces for better osteointegration of orthopedic implants”, Journal of Materials Chemistry, vol. 22, pp. 1904-1915, 2012.