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Hierarchically designed electrospun tubular scaffolds for cardiovascular applications

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Journal of Biomedical Nanotechnology

Source : Journal of Biomedical Nanotechnology, Volume 7, Number 5, p.609-620 (2011)

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Keywords : 6 diamidino 2 phenylindole, Acetylation, Adhesion, article, Bi-layer, Biological materials, Biomaterials, blood vessel prosthesis, Cardiovascular, Cardiovascular applications, cell adhesion, cell interaction, cell motion, Cell Movement, cell proliferation, cell shape, Cellular interaction, Cellular orientation, chemistry, Confocal, confocal microscopy, Cytology, Cytoskeletal organization, cytoskeleton, differential scanning calorimetry, Differential thermal analysis, drug effect, Electron, Electrospinning, Electrospinning techniques, Electrospun nanofibers, Electrospuns, Endothelial cells, Fibers, Fluorescence, fluorescence microscopy, fluorescent antibody technique, fluorescent dye, Fluorescent Dyes, Fourier Transform Infrared, Fourier transform infrared photoacoustic spectroscopy, Fourier transform infrared spectroscopy, FTIR spectroscopy, human, human cell, Human umbilical vein endothelial cells, Humans, In-vivo, indole derivative, Indoles, infrared spectroscopy

Campus : Kochi

School : Center for Nanosciences

Center : Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2011

Abstract : Hierarchically designed tubular scaffolds with bi-layer and multi-layer structures are expected to mimic native vessels in its structural geometry. A new approach for the fabrication of hierarchically designed tubular scaffold with suitable morphology was introduced through electrospinning technique. Among these scaffolds, bi-layer scaffold had a single inner and outer layer whereas multilayer scaffold had more number of inner layers. The inner layer/layers of the scaffolds were made up of aligned poly (lactic acid) (PLA) fibers for EC adhesion where as outer layers were composed of random fibers of poly (caprolactone) (PCL) and PLA providing larger pores for SMC penetration. The fabricated scaffolds were characterized by FTIR spectroscopy and Differential Thermal Analysis (DTA) and examined by evaluating cellular interactions. Human Umbilical Vein Endothelial Cells (HUVECs) seeded on aligned PLA fibers showed enhanced cellular orientation and cytoskeletal organization. In addition, the PCL-PLA composite random fibers supported SMC adhesion and proliferation sufficiently. The functionality of the endothelial cells grown on the PLA-aligned scaffold was also found to be satisfactory. Lining the constructs with a luminal monolayer of well-organized ECs along with homogenously distributed SMCs surrounding them might result in vascular conduits suitable for in vivo applications. Since this hierarchically designed tubular scaffold closely mimics the morphology of native vessel, this could be a better candidate for vascular tissue engineering. Copyright © 2011 American Scientific Publishers All rights reserved.

Cite this Research Publication : K. T. Shalumon, Sreerekha, P. R., Sathish, D., Tamura, H., Nair, S. V., Chennazhi, K. P., and Dr. Jayakumar Rangasamy, “Hierarchically designed electrospun tubular scaffolds for cardiovascular applications”, Journal of Biomedical Nanotechnology, vol. 7, pp. 609-620, 2011.

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