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Highly crystalline cotton spinning wastes utilization: Pretreatment, optimized Hydrolysis and Fermentation using Pleurotus lorida for Bioethanol production

Publication Type : Journal Article

Publisher : Elsevier

Source : Elsevier

Url :

Campus : Coimbatore

School : School of Agricultural Sciences

Year : 2022

Abstract : Due to highly deleterious effects of physico-chemical pretreatment of lignocellulosic biomass to environment, the 2G biofuel production requires more sustainable and eco-friendly approach. During this study, the effect of biological pretreatment on fibrous cotton spinning waste mixture (CSWM) was investigated using solid-state cultivation of white-rot fungi, Pleurotus florida. The pretreatment with P. florida showed significant amount of lignin degradation (∼60 %w/w) and ∼80–85% of cellulose crystallinity reduction with the cellulose content of 80.7%w/w, after 14 days of pretreatment. This was also evidenced by Attenuated Total Resonance – Fourier Transform Infrared (ATR-FTIR) spectroscopy and Scanning Electron Microscopy (SEM). Optimization of hydrolysis conditions using Central Composite Design (CCD) resulted in the R-squared (R2) value and desirability of 0.97 and 0.95, respectively. The optimized conditions were biomass loading of 0.67%w/v and enzyme loading of 60 FPU/g of cellulose results in hydrolysis efficiency of ∼70% after 96 h. The hydrolysate, supplemented with mineral salts and 3 %v/v of inoculum, were maintained at anaerobic conditions with 32 ± 2℃ for 72 h. Optimum ethanol yield of 64 % (1.18 g/L) was obtained after 60 h of fermentation. This finding suggests that microbial pretreatment can effectively be employed for the sustainable and green production of bioethanol with more economical value.

Cite this Research Publication : Ranjithkumar, M., Uthandi, S., Kumar, P. S., Muniraj, I., Thanabal, V., & Rajarathinam, R., "Highly crystalline cotton spinning wastes utilization: Pretreatment, optimized hydrolysis and fermentation using Pleurotus lorida for bioethanol production," Renewable Energy, Fuel 308(1):122052, DOI:10.1016/j.fuel.2021.122052, Elsevier, 2022.

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