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Impact of poly(lactic-co-glycolic acid) nanoparticle surface charge on protein, cellular and haematological interactions

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Colloids and Surfaces B: Biointerfaces

Source : Colloids and Surfaces B: Biointerfaces, Elsevier, Volume 136, p.1058-1066 (2015)

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Keywords : Biochemistry, Cell uptake, Charged particles, Ethylene, Nanoparticle interaction, Nanoparticle surface charge, Nanoparticles, Plasma protein adsorption, PLGA nanoparticles, Poly lactic-co-glycolic acid, Polymeric nanoparticles, Polyvinyl alcohols, Protein Binding, Proteins, surface charge

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2015

Abstract : The initial interactions of nanoparticles with biomolecules have a great influence on its toxicity, efficacy, biodistribution and clearance. The present work is an attempt to understand the impact of surface charge of polymeric nanoparticles on its plasma protein and cellular interactions. Negative, near-neutral and positively charged poly(lactic-. co-glycolic acid) [PLGA] nanoparticles were prepared using casein, poly(vinyl alcohol) and poly(ethylene imine) respectively, as surface stabilizers. A significant temporal variation in the hydrodynamic diameter of PLGA nanoparticles was observed in the presence of plasma proteins, which correlated with the amount of proteins adsorbed to each surface. Positively charged particles displayed the maximum size variation and protein adsorption. Cellular uptake of differentially charged nanoparticles was also concurrent with the quantity of adsorbed proteins, though there was no significant difference in their cytotoxicity. Haematological interactions (haemolysis and plasma coagulation times) of positively charged nanoparticles were considerably different from near-neutral and negative nanoparticles. Collectively, the results point to the interplay between plasma protein adsorption and cellular interactions of PLGA nanoparticles, which is governed by its surface charge, thereby necessitating a rational design of nanoparticles. © 2015 Elsevier B.V.

Cite this Research Publication : G. J. Pillai, Greeshma, M. M., and Dr. Deepthy Menon, “Impact of poly(lactic-co-glycolic acid) nanoparticle surface charge on protein, cellular and haematological interactions”, Colloids and Surfaces B: Biointerfaces, vol. 136, pp. 1058-1066, 2015.

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