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In Vitro Targeted Imaging and Delivery of Camptothecin using Cetuximab-Conjugated Multifunctional PLGA-ZnS Nanoparticles

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : NanomedicineNanomedicine,

Source : Nanomedicine, Volume 7, Number 4, p.507-519 (2012)

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Keywords : Antibodies, article, camptothecin, Cell Line, cell viability, Cetuximab, controlled release formulation, controlled study, cytotoxicity, drug activity, Drug Carriers, drug conjugation, drug delivery system, drug release, drug targeting, epidermal growth factor receptor, Hemolysis, human, human cell, Humans, in vitro study, Lactic acid, Monoclonal, nanomedicine, nanoparticle, Nanoparticles, Nanotechnology, physical chemistry, polyglactin, polyglycolic acid, priority journal, protein expression, quantum dot, Sulfides, sustained drug release, Tumor, Zinc compounds, zinc sulfide

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2012

Abstract : Background: Targeted cancer therapy has been extensively developed to improve the quality of treatment by reducing the systemic exposure of cytotoxic drug. Polymeric nanoparticles with conjugated targeting agents are widely investigated because they offer tunability in particle size, drug release profile and biocompatibility. Materials amp; methods: Here, we have prepared targeted multifunctional nanoparticles composed of a poly(lactic-co-glycolic acid) matrix, ZnS:Mn 2+ quantum dots and camptothecin, and targeted them to EGF receptor overexpressing cells with a cetuximab antibody. Results: Physicochemical characterization of multifunctional nanoparticles showed stable particles with sizes of lt;200 nm. In vitro drug release and blood contact studies showed a sustained release profile, with limited hemolysis. In vitro cytotoxicity and cell uptake studies were carried out in A549, KB and MFC-7 cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, FACS, fluorescent microscopic images and spectroflourimetry. Conclusion: Our studies revealed higher camptothecin activity and uptake in cell lines that overexpress the EGF receptor. All these results suggest that anti-EGF receptor cetuximab-conjugated poly(lactic-co-glycolic acid) multifunctional nanoparticles can be used as a potential nanomedicine against cancer. © 2012 Future Medicine Ltd.

Cite this Research Publication : V. G. Deepagan, Sarmento, B., Dr. Deepthy Menon, Nascimento, A., Jayasree, A., Sreeranganathan, M., Dr. Manzoor K., Nair, S. V., and Dr. Jayakumar Rangasamy, “In Vitro Targeted Imaging and Delivery of Camptothecin using Cetuximab-Conjugated Multifunctional PLGA-ZnS Nanoparticles”, Nanomedicine, vol. 7, pp. 507-519, 2012.

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