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Injectable Deferoxamine Nanoparticles Loaded Chitosan-hyaluronic Acid Coacervate Hydrogel for Therapeutic Angiogenesis

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Colloids Surf B Biointerfaces

Source : Colloids Surf B Biointerfaces, Volume 161, p.129-138 (2018)

Keywords : Animals, Cells, Cultured, chitosan, Deferoxamine, Delayed-Action Preparations, Drug Liberation, Human umbilical vein endothelial cells, Humans, hyaluronic acid, Hydrogel, Polyethylene Glycol Dimethacrylate, Injections, Lactic acid, Mice, Inbred BALB C, Nanoparticles, Neovascularization, Physiologic, particle size, polyglycolic acid, Polylactic Acid-Polyglycolic Acid Copolymer

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences, Nanosciences and Molecular Medicine

Year : 2018

Abstract : In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis. DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties. The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%. An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared. Both free DFO and DFO NPs were entrapped into the prepared CS-HA composite hydrogel. The hydrogels were characterized by SEM, FTIR and Rheology. Addition of DFO NPs did not affect the injectablility and flowability of developed hydrogels. In vitro DFO release from the prepared composite hydrogels showed controlled release over a period of 10days. Both the hydrogel systems showed excellent cyto-compatability and good cell proliferation for rASCs as well as HUVECs. The DFO and DFO NPs loaded composite hydrogels revealed effective tube formation in comparison with control hydrogels without DFO and DFO NPs. The in vivo angiogenic evaluation of the free DFO and DFO NPs (0.025%w/w) loaded composite hydrogels were studied by injecting the developed hydrogel subcutaneously into mice for 2-4 weeks. The DFO NPs loaded composite hydrogel had enhanced neovascularization when compared to control gels. Thus, the developed DFO NPs loaded composite hydrogel could potentially be used for therapeutic angiogenesis.

Cite this Research Publication : Vignesh S., A, S., Annapoorna, M., R, J., Subramania, I., Shantikumar V Nair, and Dr. Jayakumar Rangasamy, “Injectable Deferoxamine Nanoparticles Loaded Chitosan-hyaluronic Acid Coacervate Hydrogel for Therapeutic Angiogenesis”, Colloids Surf B Biointerfaces, vol. 161, pp. 129-138, 2018.

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