Publication Type : Journal Article
Source : Indian Journal Med Microbio, 1989
Url : https://link.springer.com/article/10.1007/BF00193851
Campus : Kochi
School : School of Medicine
Abstract : Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.
Cite this Research Publication : Annamma M ,Radhakrishnan V.V, Rao SB. Isolation of Mycobacterium tuberculosis antigen 5 by immunoabsorbent affinity chromatography and its diagnostic application in tuberculous meningitis. Indian J Med Microbio, 1989;7:49-53.