ProgramsView all programs
From the news
- Chancellor Amma Addresses the Parliament of World’s Religions
- Amrita Students Qualify for the European Mars Rover Challenge
Publication Type : Journal Article
Thematic Areas : Medical Sciences, Nanosciences and Molecular Medicine
Publisher : Journal of Integrated OMICS
Source : Journal of Integrated OMICS, Proteomass Scientific Society, Volume 8, Number 1, p.66-73 (2018)
Campus : Kochi
School : Center for Allied Health Sciences, Center for Nanosciences, School of Medicine
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2018
Abstract : The aggressiveness of brain tumors is attributed to the expression of multiple oncogenes involved in proliferation, metabolism and therapeutic resistance whose potential correlation with tumor progression has not been well-studied. In this study, we aimed to investigate the relationship of oncotargets involved in pathogenesis with respect to glioma grades. Gliomas (n=40) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing for the detection of epidermal growth factor receptor (EGFR) mutants. Expressions levels of EGFRy EGFR variant III (EGFRvIII), Lek/Yes novel tyrosine kinase (Lyn), Spleen tyrosine kinase (SYK), insulin receptor substrate 1 (IRSl)y phosphatidylinositol 3-kinase (PI3K), Src homology 2 domain-containing inositol 5-phosphatase 1 (SHIP1) and glucose transporter 3 (GLUT3) were studied using real-time PCR and compared against glioma grades via statistical methods. Protein expressions were analyzed using immunohistochemistry and western blotting. EGFRvIII was detected in 53% and exon 4 deletion (de4 EGFR) in 20% of gliomas. Importantly, the expressions levels of candidate oncogenes were significantly upregulated (Plt;0.05) and positively correlated with the glioma grades. Hence, these oncotargets require high surveillance during tumor progression and further investigations on larger patient cohorts can affirm their role as potential markers in the pathology of glioma, thereby aiding in the development of patient-specific multi-targeted therapy. © 2018, Proteomass Scientific Society. All rights reserved.
Cite this Research Publication : Serene Xavier, Karimassery Ramaiyer Sundaram, Lalitha Biswas, Dilip Panikar, Bindhu Mangalath Rajamma, Nair, S., Krishnakumar N. Menon, and Dr. Lakshmi Sumitra, “Molecular Analysis of Oncogene Expressions in Different Grades of Gliomas”, Journal of Integrated OMICS, vol. 8, pp. 66-73, 2018.