Publication Type : Journal Article
Publisher : Analytical and bioanalytical chemistry
Source : Analytical and bioanalytical chemistry, 2021
Keywords : Dopamine; Electrochemical; Murexide; Serotonin; Simultaneous; Tryptamine.
Campus : Amritapuri
School : School of Arts and Sciences
Center : Research & Projects
Department : Chemistry
Verified : Yes
Year : 2021
Abstract : This work highlights the protocol employed for the simultaneous electroanalysis of tryptamine, serotonin and dopamine using a conducting poly-murexide-based electrode. To date, this is the first-of-its-kind report of simultaneous electrochemical determination of these three targets. Features of the developed electrode were identified by employing FE-SEM analysis. Under optimized conditions, the analytes underwent an irreversible electro-oxidation at the modified electrode surface, with a linear range of 0.5-40 μΜ, 0.4-40.4 μΜ and 0.5-40 μΜ for dopamine, serotonin and tryptamine, respectively. The electrolytic medium employed for the sensing was a phosphate-buffered solution with pH 7. The specificity of the developed electrode was also satisfactory in the presence of other biomolecules including L-phenylalanine, L-serine, glucose and ascorbic acid. Thus, the developed murexide-derived conducting-polymer-based electrode was used for the simultaneous sensing of the neurochemicals dopamine, serotonin and tryptamine. Electroanalysis was also demonstrated for these targets in human serum.