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Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Acta Biomaterialia
Source : Acta Biomaterialia, Volume 8, Number 5, p.1976-1989 (2012)
Keywords : alkaline phosphatase, animal experiment, animal tissue, Animals, article, Bone regeneration, cell adhesion, cell proliferation, collagen, controlled study, decorin, enzyme activity, femur, fibronectin, gene expression, implant, in vitro study, in vivo study, male, Materials Testing, nanomaterial, Nanostructures, nonhuman, osteoblast, osteocalcin, Osteogenesis, priority journal, Prostheses and Implants, protein expression, protein fak, protein synthesis, rat, Rats, Rattus, signal transduction, Sprague-Dawley, steroid receptor coactivator, Surface properties, Titanium, transcription factor, transcription factor RUNX2, unclassified drug, upregulation, vinculin, vitronectin
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2012
Abstract : An important aspect of orthopedic implant integration is the enhancement of functional activity of osteoblasts at the tissue-implant interface without any fibrous tissue intervention. Nanostructured implant surfaces are known to enhance osteoblast activity. Previously, we have reported a simple hydrothermal method for the fabrication of non-periodic nanostructures (nanoscaffold, nanoleaves and nanoneedles) on titanium implants showing good biocompatibility and a distinct osteoblast response in vitro in terms of osteoblast adhesion to the surface. In the present work, these nanostructures have been evaluated for their detailed in vitro cellular response as well as in vivo osteointegration. Our studies showed that a specific surface nanomorphology, viz. nanoleaves, which is a network of vertically aligned, non-periodic, leaf-like structures with thickness in the nanoscale, provided a distinct increase in osteoblast cell proliferation, alkaline phosphatase (ALP) activity and collagen synthesis compared to several other types of nanomorphology, such as nanotubes, nanoscaffold and nanoneedles (rods). Gene expression analysis of ALP, osteocalcin, collagen, decorin and Runx2 showed ∼20- to 40-fold up-regulation on the leaf-like topography. Cytoskeletal arrangement studies on this substrate again revealed a unique response with favorable intracellular protein expressions of vinculin, FAK and src. In vivo osteointegration study over 12 weeks on rat model (Sprague-Dawley) showed early-stage bone formation (60% bone contact by week 2 and ∼85% by week 8, p 0.01) in the leaf-like nanopattern, without any inflammatory cytokine production. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Cite this Research Publication : V. V. Divya Rani, Vinoth-Kumar, L., Anitha, V. C., Manzoor, K., Deepthy, M., and Shantikumar, V. N., “Osteointegration of titanium implant is sensitive to specific nanostructure morphology”, Acta Biomaterialia, vol. 8, pp. 1976-1989, 2012.