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Phlorotannins from Padina tetrastromatica: structural characterisation and functional studies

Publication Type : Journal Article

Publisher : Journal of Applied Phycology, Volume 31:3131–41, 2019

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Campus : Amritapuri

School : School of Biotechnology

Year : 2019

Abstract : In this study, LC–MS/MS-based structural characterisation of phlorotannins from Padina tetrastromatica, a marine brown macroalga collected from South-West coastal region of Kerala, and its bioactivities are presented. The tandem mass spectrometric data revealed a series of phlorotannins with degree of polymerisation ranging from 2 to 18. The characteristic neutral loss of tandem mass spectra further confirmed that these molecules belong to fucophlorethol class of phlorotannins. DPPH assay of the HPLC-purified, phlorotannin-enriched fraction possesses significant free radical-scavenging activity. Cell viability assay indicated that phlorotannin concentration ranging from 1.5 to 50.0 μg mL−1 is non-toxic to THP-1 cell lines. Anti-inflammatory assay performed through gelatin zymography confirmed that phlorotannins ameliorated high-glucose-induced pro-MMP-9 expression in a dose-dependent manner whereas the level of pro-MMP-2 remains unaltered. The antimicrobial assays carried out using both the crude and HPLC-purified phlorotannin fraction showed its anti-MRSA potential.

Cite this Research Publication : Divya Nair, Muralidharan Vanuopadath, Akshaya Balasubramanian, Akshay Iyer, Shankar Ganesh, Abhishek Nair Anil, Vijesh Vikraman, Prasanth Pillai, Chinchu Bose, Bipin Gopalakrishnan Nair, Jayashree Gopalakrishna Pai & Sudarslal Sadasivan Nair, "Phlorotannins from Padina tetrastromatica: structural characterisation and functional studies.",Journal of Applied Phycology, Volume 31, 3131–3141 (2019).

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