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Polycomb preferentially targets stalled promoters of coding and non-coding transcripts

Publication Type : Journal Article

Publisher : Genome Research

Source : Genome Research, CSH Press, Volume 21, Number 2, p.216–226 (2011)

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Campus : Amritapuri, Bengaluru

School : School of Engineering

Center : Biotechnology

Year : 2011

Abstract : The Polycomb group (PcG) and Trithorax group (TrxG) of proteins are required for stable and heritable maintenance of repressed and active gene expression states. Their antagonistic function on gene control, repression for PcG and activity for TrxG, is mediated by binding to chromatin and subsequent epigenetic modification of target loci. Despite our broad knowledge about composition and enzymatic activities of the protein complexes involved, our understanding still lacks important mechanistic detail and a comprehensive view on target genes. In this study we use an extensive data set of ChIP-seq, RNA-seq, and genome-wide detection of transcription start sites (TSSs) to identify and analyze thousands of binding sites for the PcG proteins and Trithorax from a Drosophila S2 cell line. In addition of finding a preference for stalled promoter regions of annotated genes, we uncover many intergenic PcG binding sites coinciding with nonannotated TSSs. Interestingly, this set includes previously unknown promoters for primary transcripts of microRNA genes, thereby expanding the scope of Polycomb control to noncoding RNAs essential for development, apoptosis, and growth.

Cite this Research Publication : D. Enderle, Beisel, C., Stadler, M. B., Gerstung, M., Dr. Prashanth Athri, and Paro, R., “Polycomb preferentially targets stalled promoters of coding and non-coding transcripts”, Genome Research, vol. 21, pp. 216–226, 2011.

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