Publication Type : Conference Proceedings
Source : Proceedings of DAE-BRNS Symposium on Nuclear and Radiochemistry (NUCAR 2009), Board of Research in Nuclear Sciences, Department of Atomic Energy, Mumbai (India); 679 p; 2009; p. 553-554; Mumbai (India); 7-10 Jan 2009.
Url : https://inis.iaea.org/search/search.aspx?orig_q=RN:40097038
Campus : Amritapuri
School : School for Sustainable Futures
Year : 2009
Abstract : The enzyme, nucleoside diphosphate kinase (NDPK) has been purified from aqueous extract of beef liver mitochondria by hydroxylapatite chromatography followed by Gel filtration technique. This enzyme catalyzes the transphosphorylation reactions between a number of nucleoside di- and triphosphates. Commercially, pure enzyme is presently unavailable for the production of labeled nucleotides. The purification procedure reported in the present study was found to be superior to the earlier reported DEAE-cellulose ion exchange chromatography method. The purified enzyme has been successfully used to synthesize α- 32P dCTP, α- 32P CTP, α- 32P UTP, α - 33P dCTP and α - 33P UTP.
Cite this Research Publication : Vinod, V.T.P, "Potential application of beef liver nucleoside diphosphate kinase in the synthesis of 32P and 33P labeled biomolecules", Proceedings of DAE-BRNS Symposium on Nuclear and Radiochemistry (NUCAR 2009), Board of Research in Nuclear Sciences, Department of Atomic Energy, Mumbai (India); 679 p; 2009; p. 553-554; Mumbai (India); 7-10 Jan 2009.