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Sequential layer-by-layer electrospinning of nano SrCO3/PRP loaded PHBV fibrous scaffold for bone tissue engineering

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Composites Part B: Engineering

Source : Composites Part B: Engineering, Elsevier Ltd, Volume 99, p.445-452 (2016)

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Keywords : Bone, cell proliferation, Electrospinning, Fourier transform infrared spectroscopy, Osteoconduction, Osteoconductive properties, Osteogenic differentiation, Osteoinduction, Plasma (human), Platelet rich plasma, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), Scaffolds (biology), scanning electron microscopy, Spinning (fibers), Strontium, Strontium carbonate, Synthesis (chemical), tissue engineering, X ray diffraction analysis, X-ray diffraction analyses (XRD)

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences, Nanosciences and Molecular Medicine

Year : 2016

Abstract : Development of scaffolds with a blend of osteoinductive and osteoconductive properties is believed to be an effective approach towards bone regeneration. In our current research, a biodegradable Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) [PHBV]/nano strontium carbonate/Platelet Rich Plasma (PRP) composite scaffold was fabricated using sequential layer-by-layer electrospinning method. The synthesized nSrCO3 nanoparticles were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), and further the developed electrospun scaffolds were taken for in vitro assessments. Fiber diameter of the composite fibrous scaffold ranges from 400 to 800 nm. Cell proliferation analysis signifies the role of PRP in the developed scaffold. Osteogenic differentiation of hMSCs was confirmed by measuring the ALP concentration and mineral deposition on the scaffolds and demonstrates considerable enhancement on the composite scaffold. These preliminary results demonstrate that the developed electrospun biocomposite scaffold could serve as a better platform for bone regeneration.

Cite this Research Publication : K. M. Sajesh, Kiran, K., Shantikumar V Nair, and Dr. Jayakumar Rangasamy, “Sequential layer-by-layer electrospinning of nano SrCO3/PRP loaded PHBV fibrous scaffold for bone tissue engineering”, Composites Part B: Engineering, vol. 99, pp. 445-452, 2016.

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