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Synthesis, characterization and preliminary in vitro evaluation of PTH 1-34 loaded chitosan nanoparticles for osteoporosis

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Journal of Biomedical Nanotechnology

Source : Journal of Biomedical Nanotechnology, Volume 8, Number 1, p.98-106 (2012)

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Keywords : 3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide, AFM, alkaline phosphatase, animal cell, Animals, article, atomic force microscopy, Biochemistry, Bone Neoplasms, Cell Line, chitosan, chitosan nanoparticle, Chitosan nanoparticles, Coagulation, controlled study, cytotoxicity, Diseases, drug bioavailability, Drug Carriers, drug half life, drug stability, drug synthesis, Dynamic light scattering, embryo, enzyme linked immunosorbent assay, gelation, Haemolysis, Hemolysis, human, human cell, Humans, in vitro study, In-vitro, In-vitro evaluation, infrared spectroscopy, L-Lactate Dehydrogenase, light scattering, Mice, mouse, Multiple routes, nanoparticle, Nanoparticles, NIH 3T3 Cells, nonhuman, Osteoblasts, osteoporosis, parathyroid hormone, Parathyroid hormones, parathyroid hormone[1-34], particle size, Patient treatment, Peptide delivery, Peptide release, Peptides, pH, Phosphatases, quantitative analysis, rat, scanning electron microscopy, Synthesis (chemical), Tumor

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2012

Abstract : Human Parathyroid hormone 1-34 (PTH1-34) loaded chitosan nanoparticles (PTH 1-34 chitosan nanoparticles) via simple ionic gelation technique were prepared which can improve the bioavailability and half-life of the peptide. Chitosan nanoparticles and PTH 1-34 chitosan nanoparticles were synthesised and characterized by DLS, SEM, AFM, FT-IR and TG/DTA. Chitosan nanoparticles (40-60 nm) and PTH 1-34 chitosan nanoparticles (60-80 nm) with zeta potential of +60 and +40 mV respectively were subjected to haemolysis assay and tested for agglomeration in blood. MTT and LDH was performed assay using Saos-2, UMR 106, L929, NIH3T3. The in vitro peptide release profile at pH 7.5 for 144 h was quantified using PTH 1-34 ELISA Kit. Effect of released PTH 1-34 on Saos-2 was determined with ALP and BCA assay. These preliminary results pave way for the prospective use of such a carrier for the delivery of PTH 1-34 by multiple routes for the benefit of patients undergoing treatment for osteoporosis. Copyright © 2012 American Scientific Publishers All rights reserved.

Cite this Research Publication : D. Narayanan, Anitha, A., Dr. Jayakumar Rangasamy, Nair, S. V., and Chennazhi, K. P., “Synthesis, characterization and preliminary in vitro evaluation of PTH 1-34 loaded chitosan nanoparticles for osteoporosis”, Journal of Biomedical Nanotechnology, vol. 8, pp. 98-106, 2012.

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