Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Advanced Healthcare Materials
Source : Adv Healthc Mater, Volume 6, Issue 7 (2017)
Url : https://www.ncbi.nlm.nih.gov/pubmed/28128898
Keywords : Alveolar Bone Loss, Animals, Bone regeneration, Dental Cementum, Hydrogels, Nanocomposites, periodontal ligament, Rabbits, Tissue Scaffolds
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Verified : No
Year : 2017
Abstract : A tri-layered scaffolding approach is adopted for the complete and concurrent regeneration of hard tissues-cementum and alveolar bone-and soft tissue-the periodontal ligament (PDL)-at a periodontal defect site. The porous tri-layered nanocomposite hydrogel scaffold is composed of chitin-poly(lactic-co-glycolic acid) (PLGA)/nanobioactive glass ceramic (nBGC)/cementum protein 1 as the cementum layer, chitin-PLGA/fibroblast growth factor 2 as the PDL layer, and chitin-PLGA/nBGC/platelet-rich plasma derived growth factors as the alveolar bone layer. The tri-layered nanocomposite hydrogel scaffold is cytocompatible and favored cementogenic, fibrogenic, and osteogenic differentiation of human dental follicle stem cells. In vivo, tri-layered nanocomposite hydrogel scaffold with/without growth factors is implanted into rabbit maxillary periodontal defects and compared with the controls at 1 and 3 months postoperatively. The tri-layered nanocomposite hydrogel scaffold with growth factors demonstrates complete defect closure and healing with new cancellous-like tissue formation on microcomputed tomography analysis. Histological and immunohistochemical analyses further confirm the formation of new cementum, fibrous PDL, and alveolar bone with well-defined bony trabeculae in comparison to the other three groups. In conclusion, the tri-layered nanocomposite hydrogel scaffold with growth factors can serve as an alternative regenerative approach to achieve simultaneous and complete periodontal regeneration.
Cite this Research Publication : S. Sowmya, Mony, U., Jayachandran, P., Reshma, S., R Kumar, A., Arzate, H., Nair, S. V., and Jayakumar, R., “Tri-Layered Nanocomposite Hydrogel Scaffold for the Concurrent Regeneration of Cementum, Periodontal Ligament, and Alveolar Bone.”, Adv Healthc Mater, vol. 6, no. 7, 2017.