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Course Detail

Course Name Analytical Biochemistry
Program BSc. in Biotechnology, BSc. in Microbiology
Credits Three
Year Taught 2019


  • Methods of protein extraction, Protein quantitation: Biuret, Lowry, BCA and Bradford methods, Protein precipitation: Salting-in, Salting-out, Effect of organic solvents and polymers. Protein separation: Dialysis, Ultrafiltration, Centrifugation.
  • Chromatography: Partition coefficient, Retention, Resolution, Capacity factor, theoretical plate, van Deemter curve, Gel flitration chromatography, Ion exchange chromatography, Affinity chromatography, Hydrophobic interaction chromatography, Hydroxyapatite chromatography, Paper chromatography, Thin layer chromatography, Reversed-phase chromatography, Fundamentals of high performance chromatography.
  • Electrophoresis: Native PAGE, SDS-PAGE, Isoelectric focussing, 2D-PAGE, Capillary electrophoresis.
  • Spectroscopy: Fundamentals of UV Spectroscopy, Spectrophotometer, Fundamentals of fluorescence spectroscopy, Spectrofluorometer.
  • Mass Spectrometry: Electron Impact, Chemical Ionization and Fast atom Bombardment mass spectrometers.
Syllabus - Year Taught
2015 2014

Text Books


  1. Biochemistry: Voet and Voet
  2. Protein Purification Techniques: Simon Roe
  3. Protein Purification: Robert K. Scopes
  4. Physical Biochemistry: David Sheehan
  5. Practical Biochemistry: Keith Wilson and John Walker
  6. Mass Spectrometry for Biotechnology: Gary Siuzdak


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