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Course Detail

Course Name Cell & Molecular Biology – Lab
Course Code BIO 488
Program M. Sc. in Bioinformatics
Semester Two
Credits Two
Year Taught 2019


Isolation of plasmid DNA and genomic DNA; Protein extraction and purification by affinity chromatography and analysis by SDS-PAGE. Polymerase chain reaction (PCR). Microscopic observation of different types of cells: bacteria, animal cell, plant cell, fungi. Cell viability test by Trypan Blue. Image analysis (SDS-PAGE, cell morphology, tissue section) by ImageJ. Basics of animal cell culture.

Text Books


1.Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).

2.Amrita University Virtual Lab

3.Freshney, R. I. (2005). Culture of specific cell types. John Wiley & Sons, Inc.

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