Back close

Course Detail

Course Name Cell & Molecular Biology – Lab
Course Code BIO 488
Program M. Sc. in Bioinformatics
Semester Two
Credits Two
Year Taught 2019

Syllabus

Isolation of plasmid DNA and genomic DNA; Protein extraction and purification by affinity chromatography and analysis by SDS-PAGE. Polymerase chain reaction (PCR). Microscopic observation of different types of cells: bacteria, animal cell, plant cell, fungi. Cell viability test by Trypan Blue. Image analysis (SDS-PAGE, cell morphology, tissue section) by ImageJ. Basics of animal cell culture.

Text Books

Resources

1.Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).

2.Amrita University Virtual Lab

3.Freshney, R. I. (2005). Culture of specific cell types. John Wiley & Sons, Inc.

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now