| Course Name | Cell & Molecular Biology – Lab |
| Course Code | BIO 488 |
| Program | M. Sc. in Bioinformatics |
| Semester | Two |
| Credits | Two |
| Year Taught | 2019 |
Isolation of plasmid DNA and genomic DNA; Protein extraction and purification by affinity chromatography and analysis by SDS-PAGE. Polymerase chain reaction (PCR). Microscopic observation of different types of cells: bacteria, animal cell, plant cell, fungi. Cell viability test by Trypan Blue. Image analysis (SDS-PAGE, cell morphology, tissue section) by ImageJ. Basics of animal cell culture.
1.Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).
2.Amrita University Virtual Lab
3.Freshney, R. I. (2005). Culture of specific cell types. John Wiley & Sons, Inc.
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