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Course Detail

Course Name Immunology Lab
Course Code 22MMD583
Program M. Sc. Molecular Medicine(MLM)
Semester 2
Credits 2
Campuses Faridabad, Kochi

Syllabus

Practicals

(1) Bacterial and fungal culture medium preparation,

(2) Pure culture technique (eg, streaking and sub culturing),

(3) Gram staining, Fungal staining and Motility assay.

(4) Bacterial conjugation, transduction and transformation

(5) Bacterial growth curve by measuring turbidity and viable count

(6) Antibiotic sensitivity assay, MIC and MBC determination

(7) Phage titration assay

(8) Biofilm assay, MBIC and MBEC determination

(9) Ames test

(10) Giemsa staining of blood smear

(11) Blood grouping

(12) Double diffusion assay

(13) Immuno-electrophoresis

(14) Radial immunodiffusion

(15) IgG purification

(16) ELISA

Summary

Pre-requisites: Basic understanding of biology and biochemistry and immunology

Total number of classes:30

Preamble:

The students will be familiarized with the basics as well as advanced methods in microbiology and immunology that can be used for disease diagnosis. Provide hands-on training for isolation of microbes in pure culture and antibiotic

susceptibility assays. Provide the students with practical skills on lymphocyte isolation and analysis.

Course Outcomes

CO1 Provide the students the knowledge about practical skills on basic microbiology.

CO2 Students will learn about different antimicrobial activity assays.

CO3 Students will develop an understanding about components of immune system and their function.

CO4 The students will learn about mutagenic assays, biofilm assays and blood grouping.

CO5 The students will learn about different antigen and antibody interaction assays.

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
C O
CO 1 3 3 1 1 1 1 1 1 3
CO 2 3 3 1 1 1 1 1 3
CO 3 3 1 1 1 1       1   3
CO 4 3 3 1 1 1 1 3
CO 5 3 3 1 1 1 1 1 1 3

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10
C O
CO 1 3 1 2 2 3 1
CO 2 3 1 2 1
CO 3 3 1 1 2 1
CO 4 3 1 2
CO 5 3 3 2 2

Evaluation Pattern: 30+70 = 100

Internal Assessment – 30% 
Records  Evaluation  30% 
  30%
End Semester Examination – 50% 
Practical Exam  60% 
Assessment Viva  10%
  70%
Total 100%

Textbooks

  1. Practical Handbook of Microbiology- Lorrence H Green, Emanuel Goldman by CRC Press, 4th Edition, ISBN 9780367567637
  2. Practical Immunology-Frank C. Hay, Olwyn M.R. Westwood, Fourth Edition,Blackwell Science.

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