Unit 1

Introduction To Lab-On-Chip the Diffusion of Molecules and Microscale Mixing, Technological Production of Components: Mixers and Pumps, Separation, Purification, Concentration Technologies, Simulation and Design of Mixing Devices for Chemical Reactors, Design and Simulation of Lab-On-A-Chip Devices, A Considered Approach to Lab-On-A-Chip Fabrication, Fluidic Platforms and Components of Lab-On-A-Chip Devices.

Unit 2

Fabrication of Lab-On-Chip Products DC Fields In Microsystems: Electro-Osmosis and Electrophoresis, AC Fields In Microsystems: Spectroscopy and Dielectrophoresis, Soft Lithography, Novel Methods and Fabrication of Lab-On-A-Chip Devices, Detection Methods – Electrical, Optical, Thermal, Applications of Paper-Based Diagnostics, Microfluidics in Planar Microchannels: Synthesis of Chemical Compounds On-Chip.

Unit 3

Molecular Biology on A Chip Sample Preparation: Fluid Conditioning for Cell and Cell Free Analysis; Microfluidic Immunoassay: Pregnancy Test, Homogeneous Phase Immunoassays, Heterogeneous Phase; Chips for Genomics and Proteomics: Microarray and DNA Based Molecules, Automated DNA Purification, Microfluidic Cdna Synthesizer, PCR Chips, Protein Immunoblotting on Chip, Protein Crystallization Chip; Electrospray Mass Spectrometry; Biochemical Analysis Using Force Sensors.

Unit 4

Cell-Based Chip for Biotechnology Microfluidic Flow Cytometers; Cell Sorting: RBC Assays, Electrokinetic Routing of Cells, Dean Flow in Spiral Microchannels, Cell Sorting Using Surface Acoustic Waves; Cell Trapping: Neuro Cages, PEG Microwells, PDMS Microwells, Dielectrophoretic Trap, Micromagnetic Traps, Hydrodynamic Traps, Trapping Cells Using Antibodies, Microdroplets Culture and Assays; Microfluidic Cell Culture Laboratory; Micro Bioreactors; Patch Clamp Chips.

Course Objectives:

• To Make Students Understand the Basic Concepts of Lab-On-Chip Architecture.
• To Create Problem Solving Ability Among Students for On-Chip Solution Development.
• To Encourage Students for Designing Lab-On-Chip Healthcare Products.
• To Prepare Students for Advance Level Courses Lab on Chip Fabrication Technology.

Course Outcomes:

After completing this course, students should be able to
CO1: Design and simulate Lab-On-A-Chip devices, considering fluidic platforms and components.
CO2: Demonstrate proficiency in the fabrication of Lab-On-Chip products, employing techniques such as soft lithography and utilizing DC and AC fields for microsystems.
CO3: Apply molecular biology techniques on a chip, including sample preparation, microfluidic immunoassays, and chips for genomics and proteomics.
CO4: Utilize cell-based chips for biotechnological applications, including microfluidic flow cytometers, cell sorting, cell trapping, and microfluidic cell culture.
CO5: Perform biochemical analysis using force sensors, demonstrating an understanding of the integration of various detection methods, including electrical, optical, and thermal approaches, in Lab-On-Chip devices.

CO-PO Mapping

1. Oppenheim Oosterbroek and van den Berg, Lab-on-a-chip: miniaturized systems for (bio)chemical analysis and synthesis. Elsevier, 2003.
2. Marc J. Madou, Fundamentals of Microfabrication, The Science of Miniaturization. CRC Press, 2002.
3. Tabeling, Introduction to Microfluidics. Oxford, 2005.
4. Nguyen and Wereley, Fundamentals and applications of microfluidics. Artech, 2006.
5. Gescheke et al, Microsystems Engineering of Lab-on-a-Chip Devices. Wiley, 2004.