Back close

Fibronectin Purification and Fragmentation

Start Date: Sunday, Jan 01,2012

End Date: Wednesday, May 30,2012

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Dr. Sanjay Pal
Co-Project Incharge:BaluBalan Gandhi, DilipMenon. P, Najma N, Neethu A, R Rakesh
Fibronectin Purification and Fragmentation

Fibronectin, an extra cellular matrix glycoprotein was purified from human plasma obtained from Regional Cancer Centre Trivandrum. Gelatin based affinity chromatography was used for the purification of the protein. The usage of pressure was found to have prominent impact in the purification of the protein by reducing albumin contamination, which we suspect would have non specifically bound to the gelatin resin. The quality of the protein purified was analysed using SDS –PAGE. The protein sample was further dialysed to remove urea as our future experiment required pure Fibronectin.
An experiment was also done to develop angiogenesis assay model using the chorioallantoic membrane (CAM) of fertilized chicken egg. Dimethyl sulfoxide (DMSO) was found to inhibit angiogenesis and sodium nitroprusside was found to have vasodilation effect, validating our assay model. We also tried to purify fibronectin from zebrafish using two different concentrations of urea which would solubilise the insoluble cellular fibronectin.

Related Projects

Escherichia coli Based Microfluidic Whole-cell Glucose Biosensor
Escherichia coli Based Microfluidic Whole-cell Glucose Biosensor
Effects of Nisin in upsurging the adequacy of conventional antibiotics against a few enteric pathogens: An invitro study
Effects of Nisin in upsurging the adequacy of conventional antibiotics against a few enteric pathogens: An invitro study
Isolation and identification of endophytes from marine algae
Isolation and identification of endophytes from marine algae
Virtual Amrita Laboratories in Biotechnology
Virtual Amrita Laboratories in Biotechnology
Studies on Biofilm Inhibition and Antibacterial Properties of Selected Plant Extracts Against S.mutans, a Dental Plaque Pathogen
Studies on Biofilm Inhibition and Antibacterial Properties of Selected Plant Extracts Against S.mutans, a Dental Plaque Pathogen
Admissions Apply Now