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A Multi-Institutional Comparative Study of Laboratories

Publication Type : Conference Paper

Thematic Areas : Biotech, Learning-Technologies, Medical Sciences

Publisher : Proceedings of International Conference on Electrical, Electronics and Optimization Techniques (ICEEOT 2016), Chennai .

Source : Proceedings of International Conference on Electrical, Electronics and Optimization Techniques (ICEEOT 2016), Chennai (2016)

Campus : Amritapuri, Coimbatore

School : School of Engineering, Centre for Cybersecurity Systems and Networks, School of Biotechnology

Center : Computational Neuroscience and Neurophysiology, Amrita Mind Brain Center, Cyber Security, Electronics Communication and Instrumentation Forum (ECIF)

Department : biotechnology, Chemical, Computational Neuroscience Laboratory, cyber Security, Electronics and Communication

Year : 2016

Abstract : Mass spectrometry (MS) of glycoproteins is an emerging field in proteomics, poised to meet the technical demand for elucidation of the structural complexity and functions of the oligosaccharide components of molecules. Considering the divergence of the mass spectrometric methods employed for oligosaccharide analysis in recent publications, it is necessary to establish technical standards and demonstrate capabilities. In the present study of the Human Proteome Organisation (HUPO) Human Disease Glycomics/Proteome Initiative (HGPI), the same samples of transferrin and immunoglobulin-G were analyzed for N-linked oligosaccharides and their relative abundances in 20 laboratories, and the chromatographic and mass spectrometric analysis results were evaluated. In general, matrix-assisted laser desorption/ionization (MALDI) time-of-flight MS of permethylated oligosaccharide mixtures carried out in six laboratories yielded good quantitation, and the results can be correlated to those of chromatography of reductive amination derivatives. For underivatized oligosaccharide alditols, graphitized carbon-liquid chromatography (LC)/electrospray ionization (ESI) MS detecting deprotonated molecules in the negative ion mode provided acceptable quantitation. The variance of the results among these three methods was small. Detailed analyses of tryptic glycopeptides employing either nano LC/ESI MS/MS or MALDI MS demonstrated excellent capability to determine site-specific or subclass-specific glycan profiles in these samples. Taking into account the variety of MS technologies and options for distinct protocols used in this study, the results of this multi-institutional study indicate that MS-based analysis appears as the efficient method for identification and quantitation of oligosaccharides in glycomic studies and endorse the power of MS for glycopeptide characterization with high sensitivity in proteomic programs.

Cite this Research Publication : M. S, Dr. Krishnashree Achuthan, and Dr. Shyam Diwakar, “A Multi-Institutional Comparative Study of Laboratories”, in Proceedings of IEEE International Conference on Electrical, Electronics and Optimization Techniques (ICEEOT 2016), Chennai, March 3-5, 2016.

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