Publication Type : Journal Article
Publisher : Genome Research
Source : Genome Research, Volume 21, Number 11, p.1872-1881 (2011)
Url : http://www.scopus.com/inward/record.url?eid=2-s2.0-80555142954&partnerID=40&md5=d428a21889132a9ca329642fd7c116e1
Keywords : accuracy, Alternative Splicing, Animals, Anopheles gambiae, article, Chromosome Mapping, codon, complementary DNA, exon, Exons, Fourier transform mass spectrometry, gene identification, gene sequence, gene structure, Genes, genetic code, genomics, Initiator, Insect, insect genetics, intron, Introns, mass spectrometry, Molecular Sequence Annotation, Molecular Sequence Data, nonhuman, nucleotide sequence, Open Reading Frames, Peptides, priority journal, proteomics, Reproducibility of Results, reverse transcription polymerase chain reaction, RNA Splice Sites, RNA translation, spacer DNA, translation initiation, unindexed sequence, Untranslated Regions, utricle
Campus : Amritapuri
School : School of Biotechnology
Department : biotechnology
Year : 2011
Abstract : Anopheles gambiae is a major mosquito vector responsible for malaria transmission, whose genome sequence was reported in 2002. Genome annotation is a continuing effort, and many of the approximately 13,000 genes listed in VectorBase for Anopheles gambiae are predictions that have still not been validated by any other method. To identify protein-coding genes of An. gambiae based on its genomic sequence, we carried out a deep proteomic analysis using high-resolution Fourier transform mass spectrometry for both precursor and fragment ions. Based on peptide evidence, we were able to support or correct more than 6000 gene annotations including 80 novel gene structures and about 500 translational start sites. An additional validation by RT-PCR and cDNA sequencing was successfully performed for 105 selected genes. Our proteogenomic analysis led to the identification of 2682 genome search-specific peptides. Numerous cases of encoded proteins were documented in regions annotated as intergenic, introns, or untranslated regions. Using a database created to contain potential splice sites, we also identified 35 novel splice junctions. This is a first report to annotate the An. gambiae genome using high-accuracy mass spectrometry data as a complementary technology for genome annotation. © 2011 by Cold Spring Harbor Laboratory Press.
Cite this Research Publication : Rab Chaerkady, Kelkar, D. Sbc, Muthusamy, Bbd, Kandasamy, Kab, Dwivedi, S. Bbc, Sahasrabuddhe, N. Aab e, Kim, M. - Sa, Renuse, Sab c, Pinto, S. Mbe, Sharma, Rf, Pawar, Hbg, Sekhar, N. Rbd, Mohanty, A. Kh, Getnet, Da, Yang, Ya, Zhong, Ja, Dash, A. Pi, MacCallum, R. Mj, Delanghe, Bk, Mlambo, Gl, Kumar, Ah, Prasad, T. S. Kbd e, Okulate, Mm, Kumar, Nln, and Pandey, Aao, “A proteogenomic analysis of Anopheles gambiae using high-resolution Fourier transform mass spectrometry”, Genome Research, vol. 21, pp. 1872-1881, 2011.