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Antistaphylococcal and Neutrophil Chemotactic Injectable k‑Carrageenan Hydrogel for Infectious Wound Healing

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : ACS Applied Bio Materials

Source : ACS Applied Bio Materials, Volume 2, p.378-387 (2019)

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2019

Abstract : Staphylococcus aureus wound infection is a major concern due to the resistance of S. aureus to topical antibiotics and capacity to inhibit neutrophil migration at the infection site. To overcome these problems, we have developed 0.01% (v/v) octenidine dihydrochloride (Oct) and 0.5% (w/w) chitosan-treated serum (CTS) containing 1.5% (w/v) κ-carrageenan hydrogel (κC). Oct is an antiseptic agent, against which no resistance is reported so far, and CTS has neutrophilic attractant properties. The prepared Oct-CTS-κC hydrogel is injectable and biocompatible. Using in vitro experiments, we demonstrated CTS can induce the migration of polymorphonuclear neutrophils (PMNs) and fibroblasts that can facilitate tissue regeneration at a wound site. In vitro release studies revealed a sustained release of Oct and serum proteins from the Oct-CTS-κC hydrogel. Antibacterial properties of developed hydrogels were tested against S. aureus and its clinical isolates. Further, the in vivo antibacterial efficacy of the prepared hydrogel was evaluated in an S. aureus-infected Sprague–Dawley (SD) rat wound. Both in vitro and in vivo studies showed that the Oct-CTS-κC hydrogel inhibited S. aureus growth. Thus, the developed Oct-CTS-κC hydrogel can be potentially exploited for S. aureus-infected wound healing.

Cite this Research Publication : M. Shiva, V. Aparna, Ramanathan, Y., S. Vignesh, V. Kumar, A., Dr. Jayakumar Rangasamy, and Raja Biswas, “Antistaphylococcal and Neutrophil Chemotactic Injectable k‑Carrageenan Hydrogel for Infectious Wound Healing”, ACS Applied Bio Materials, vol. 2, pp. 378-387, 2019.

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