Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : International Journal of Biological Macromolecules
Source : International Journal of Biological Macromolecules, Volume 110, p.140 - 149 (2018)
Url : http://www.sciencedirect.com/science/article/pii/S014181301733859X
Keywords : amphotericin B, Carboxymethylated ɩ-carrageenan, Gelatin A, Macrophages, Targeted drug delivery
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2018
Abstract : Intercellular Candida glabrata infections are difficult to treat due to poor penetration of drugs into the fungal niche. Delivering amphotericin B (Amp B) into the macrophages where the pathogen inhabits is an effective solution. We are studying the macrophage targeting proficiency of ɩ-carrageenan for the delivery of Amp B using gelatin A nanoparticles (GNPs). The choice of gelatin A was the outcome of in silico inspections where the amino functionalized polymer having the best docking score with Amp B was selected. We prepared a sustained release formulation of amp B loaded carboxymethyl ɩ-carrageenan conjugated gelatin nanoparticles (CMC-Amp B-GNPs) with size 343±12nm and -25±5.3mV zeta potential. The formulations were found to be stable, biocompatible and non-haemolytic. Flow cytometry analysis showed 3 fold higher uptake of CMC-GNPs compared to the GNPs by RAW 264.7 cells. CMC-Amp B-GNPs showed enhanced antifungal activity than bare Amp B and Amp B-GNPs.
Cite this Research Publication : V. Aparna, Melge, A. Rohit, Rajan, V. K., Raja Biswas, Dr. Jayakumar Rangasamy, and Dr. Gopi Mohan C., “Carboxymethylated ɩ-carrageenan Conjugated Amphotericin B loaded Gelatin Nanoparticles for Treating Intracellular Candida Glabrata Infections”, International Journal of Biological Macromolecules, vol. 110, pp. 140 - 149, 2018.