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Cellular and molecular mechanistic insight into the DNA-damaging potential of few-layer graphene in human primary endothelial cells

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Nanomedicine: Nanotechnology, Biology, and Medicine

Source : Nanomedicine: Nanotechnology, Biology, and Medicine, Elsevier Inc., Volume 12, Number 5, p.1347-1355 (2016)

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Keywords : Assays, Biomedical applications, cDNA microarray analysis, Cell culture, Cell death, Cell membranes, DNA, DNA damages, Electrophoresis, Endothelial cells, Few-layer graphene, Graphene, Medical applications, Microarrays, Mitochondria, Mitochondrial superoxide, oxidative stress, Oxygen, Primary cells, Single cell gel electrophoresis, toxicity, Umbilical vein endothelial cells

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine, Nanosciences

Year : 2016

Abstract : Despite graphene being proposed for a multitude of biomedical applications, there is a dearth in the fundamental cellular and molecular level understanding of how few-layer graphene (FLG) interacts with human primary cells. Herein, using human primary umbilical vein endothelial cells as model of vascular transport, we investigated the basic mechanism underlying the biological behavior of graphene. Mechanistic toxicity studies using a battery of cell based assays revealed an organized oxidative stress paradigm involving cytosolic reactive oxygen stress, mitochondrial superoxide generation, lipid peroxidation, glutathione oxidation, mitochondrial membrane depolarization, enhanced calcium efflux, all leading to cell death by apoptosis/necrosis. We further investigated the effect of graphene interactions using cDNA microarray analysis and identified potential adverse effects by down regulating key genes involved in DNA damage response and repair mechanisms. Single cell gel electrophoresis assay/Comet assay confirmed the DNA damaging potential of graphene towards human primary cells. © 2016 Elsevier Inc.

Cite this Research Publication : A. Sasidharan, Swaroop, S., Chandran, P., Shantikumar V Nair, and Dr. Manzoor K., “Cellular and molecular mechanistic insight into the DNA-damaging potential of few-layer graphene in human primary endothelial cells”, Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 12, pp. 1347-1355, 2016.

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