Publication

Abstract : The high prevalence of co-resistance to betalactam, aminoglycoside and quinolone against PSA has necessitated increased use of carbapenems. MBL production among PSA is one of the several mechanisms causing carbapenem resistance (CARB-R) transferable by integrons. We surveyed MBL production among PSA isolates collected via multicentric surveillance study. Methods: During March-September 2009 BMPLIII received 75 consecutively collected PSA causing infections of (skin and soft tissue-48, blood and respiratory tract 11 each, and other sites, 5) from four (50-AIMS,Kochi, 21-SGPGIMS, 2-MGIMS,2-AIIMS,Delhi) Indian Medical centers. Antimicrobial susceptibility testing by Kirby Bauer method against ceftazidime (CZD), cefepime(FEP), piperacillin/tazobactam (TZP), ticarcillin /clavulanic acid (TIM), gentamicin (GEN), amikacin(AK), ciprofloxacin(CIP), imipenem(IMP), meropenem(MEM), aztreonam(ATM) and colistin(CL) was done. MBL was screened by the Combined Disk Diffusion Test (CDDT) method using 0.5 M EDTA (930μg) as the inhibitor with IMP, positives (inhibition ≥7 mm) confirmed by IMP + EDTA Etest and PCR (VIM and IMP genes).