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Cryptococcus neoformans var. gattii isolate with Unusual Morphology

Publication Type : Journal Article

Thematic Areas : Medical Sciences

Publisher : Rev Soc Bras Med Trop,

Source : Rev Soc Bras Med Trop, 2020

Url :

Campus : Kochi

School : School of Medicine

Department : Microbiology

Year : 2020

Abstract : An immunocompetent 24-year-old woman was referred to our hospital with viral meningitis that did not respond to treatment. Brain magnetic resonance imaging (MRI) revealed signs suggestive of acute disseminated encephalomyelitis. Cerebrospinal fluid (CSF) showed glucose levels of 64.8 mg/dL and protein levels of 65.6 mg/dL. CSF microscopy showed 42 cells/mm3, including polymorphs, lymphocytes, and yeast cells with atypical morphology. Grocott-Gomori's-methenamine-silver staining, India ink staining, and calcofluor white staining of CSF demonstrated the existence of unusual morphological features like pseudohypha formation, chains of budding yeasts, and structures resembling germ tubes (Figures A, Figure B and Figure C). Most of the cells were encapsulated. Sequencing identified yeast isolates grown on blood and CSF cultures as Cryptococcus neoformansvar. gattii. The isolate was sensitive to voriconazole, amphotericin B, fluconazole, and flucytosine. The patient succumbed to the infection despite initiating treatment with liposomal amphotericin B.

Cite this Research Publication : Anil Kumar, Malini Eapen, Rosamma Philip "Cryptococcus neoformans var. gattii isolate with Unusual Morphology", Rev Soc Bras Med Trop, 2020

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