Publication Type : Journal Article
Thematic Areas : Medical Sciences
Publisher : Journal of Experimental Pathology
Source : Journal of Experimental Pathology, Volume 3, Number 1, p.75-86 (1987)
Url : http://www.scopus.com/inward/record.url?eid=2-s2.0-0023130747&partnerID=40&md5=120d676307d9aaf497360611fa1505cf
Keywords : Animals, Antigens, article, Cytology, fluorescent antibody technique, herpes antigen, herpes simplex, herpes simplex virus 1, human, human cell, Humans, Immunoenzyme Techniques, immunofluorescence, immunohistochemistry, major clinical study, mouth, mouth carcinoma, Mouth Neoplasms, Simplexvirus, unclassified drug, Vero cells, Viral
Campus : Kochi
School : School of Medicine
Department : Biochemistry
Year : 1987
Abstract : Biopsies from one hundred and seventy five patients suffering from carcinoma of the oral cavity were examined for the presence of Herpes simplex virus Type 1 (HSV - 1) antigen, using Immunofluorescence (Direct and indirect) and immunoperoxidase techniques. Seventy-seven percent of the biopsies showed significant fluorescence, while 71% showed positivity with immunoperoxidase staining. There was no correlation either in the degree of fluorescence or in the intensity of peroxidase staining with clinical stage of the disease. Normal oral biopsies showed 30% and 20% positivity in immunofluorescence and immunoperoxidase respectively. In the case of oral cancer cells, the fluorescence was very much diminished when HSV.1 antiserum was absorbed with specific homologous virus-infected Vero cells.
Cite this Research Publication : T. Vijayakumar, Kumari, T. V., Vasudevan, D. M., Ankathil, R., and Ramani, P., “Demonstration of HSV-I antigen in patients with oral cancer by immunofluorescence and immunoperoxidase techniques”, Journal of Experimental Pathology, vol. 3, pp. 75-86, 1987.