Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Journal of Materials Chemistry B, Royal Society of Chemistry,
Source : Journal of Materials Chemistry B, Royal Society of Chemistry, Volume 3, Number 28, p.5795-5805 (2015)
Url : http://www.scopus.com/inward/record.url?eid=2-s2.0-84936946007&partnerID=40&md5=f57684e70da32f67cea906a8c5224fea
Keywords : Anti-bacterial activity, Bacteria, Bacterial Infections, biocompatibility, Biomaterials, Cell culture, chitin, chitosan, Drug products, Dynamic light scattering, Escherichia coli, Fourier transform infra reds, Human dermal fibroblasts, hyaluronic acid, light scattering, Mechanical testing, Mobile security, Muscle, O-carboxymethyl chitosans, Physiochemical characterization, scanning electron microscopy, Sensitivity studies, Staphylococcus aureus, Tissue
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2015
Abstract : Excessive bleeding due to premature clot lysis and secondary bacterial wound infection are two significant problems that contribute to increased morbidity in patients with hyperfibrinolytic conditions. In this study, we have developed a bi-layered sponge that promotes fibrin clot stability and prevents secondary bacterial wound infections. Using the technique of freeze-drying, a bi-layer matrix consisting of hyaluronic acid (HA) containing aminocaproic acid (amicar) and chitosan containing tetracycline loaded O-carboxymethyl chitosan nanoparticles (Tet-O-CMC NPs) were produced. We hypothesized that the top chitosan layer with Tet-O-CMC NPs will prevent wound infection and concomitantly act as a matrix for cellular migration and subsequent wound healing, while the amicar-containing layer would promote clot stability. Tet-O-CMC NPs and bi-layer sponges were characterized using Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) and Fourier Transform Infra Red (FT-IR) spectroscopy. Physiochemical characterization such as porosity, swelling and mechanical testing was performed. The drug release study shows that the bi-layered sponge demonstrates a robust burst release of amicar and a sustained release of tetracycline. The ex vivo muscle permeation study indicated that Tet-O-CMC NPs have enhanced tissue permeation compared to free Tet. In vitro antibacterial activity of the bi-layer sponge towards laboratory and clinical strains of Staphylococcus aureus and Escherichia coli was proved. The ex vivo bacterial sensitivity study using porcine muscles confirmed the antibacterial activity, while the cell viability study using human dermal fibroblast (HDF) cells revealed its biocompatible nature. The in vitro antifibrinolytic study shows that the bi-layered sponge with amicar showed significant protection against streptokinase induced clot lysis. These studies suggest that the prepared amicar and tetracycline loaded chitosan-HA bi-layered sponge can be used effectively to promote better wound healing by simultaneously preventing bacterial infection, and enhancing clot stability. This journal is © The Royal Society of Chemistry 2015.
Cite this Research Publication : A. Mohandas, Nimal, T. R., Das, V., Dr. Sahadev Shankarappa, Dr. Raja Biswas, and Dr. Jayakumar Rangasamy, “Drug Loaded Bi-layered Sponge for Wound Management in Hyperfibrinolytic Conditions”, Journal of Materials Chemistry B, vol. 3, pp. 5795-5805, 2015.